FURA-2 protocol

eagle-i ID

Resource Type

Properties

1. Resource Description

   "Fura-2, a polyamino carboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium. It was the first widely-used dye for calcium imaging, and remains very popular. Fura-2 is excited at 340 nm and 380 nm of light, and the ratio of the emissions at those wavelengths is directly correlated to the amount of intracellular calcium. The use of the ratio automatically cancels out confounding variables, such as variable dye concentration and cell thickness, making Fura-2 one of the most appreciated tools to quantify calcium levels. More recently, genetically-encoded calcium indicators based on spectral variants of the green fluorescent protein, such as Cameleons, have supplemented the use of Fura-2 and other small molecule dyes for calcium imaging."

2. Contact

   0000012a-24ff-89c6-d994-629180000000

3. Uses

   Calcium imaging assay

4. Used by

   Imaging and Morphology Support Core Laboratory