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Protein identification and partial sequencing

eagle-i ID


Resource Type

  1. Material analysis service


  1. Fee for service
  2. Resource Description
    "Identification of unknown proteins is the bread-and-butter analysis for a Proteomics Facility. Proteins are enzymatically digested into peptides, which are then introduced into a mass spectrometer via a liquid chromatography system. Once inside the mass spectrometer we fragment the peptides to produce MS/MS spectra. These fragmentation patterns are matched against theoretical spectra from a protein database. Scoring algorithms and statistical tools are then used to determine the identity of the proteins in the sample. Samples can be submitted as either a liquid solution of the protein(s), or as a band from a gel. Liquid samples may also by lyophilized or taken to dryness before submission."
  3. Service Fee URL
  4. Contact
    David, Larry, Ph.D.
  5. Related Resource
    Thermo Fisher LTQ spectrometer
  6. Related Resource
    Thermo Fisher LTQ VELOS Pro spectrometer
  7. Related Resource
    Agilent Capillary liquid chromatography system
  8. Related Resource
  9. Related Resource
  10. Related Resource
  11. Related Resource
    Proteomic Analysis Workflow pipeline
  12. Related Resource
    Thermo Fisher LTQ VELOS mass spectrometer
  13. Service Provided by
    Proteomics Shared Resource Core Laboratory
  14. Website(s)
  15. Website(s)
  16. Related Technique
    Protein identification
  17. Related Technique
    Partial protein sequencing
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016