Protein identification and partial sequencing

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "Identification of unknown proteins is the bread-and-butter analysis for a Proteomics Facility. Proteins are enzymatically digested into peptides, which are then introduced into a mass spectrometer via a liquid chromatography system. Once inside the mass spectrometer we fragment the peptides to produce MS/MS spectra. These fragmentation patterns are matched against theoretical spectra from a protein database. Scoring algorithms and statistical tools are then used to determine the identity of the proteins in the sample. Samples can be submitted as either a liquid solution of the protein(s), or as a band from a gel. Liquid samples may also by lyophilized or taken to dryness before submission."

3. Service Fee URL

   http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/rates.cfm

4. Contact

   David, Larry, Ph.D.

5. Related Resource

   Thermo Fisher LTQ spectrometer

6. Related Resource

   Thermo Fisher LTQ VELOS Pro spectrometer

7. Related Resource

   Agilent Capillary liquid chromatography system

8. Related Resource

   SEQUEST

9. Related Resource

   Mascot

10. Related Resource

    Scaffold

11. Related Resource

    Proteomic Analysis Workflow pipeline

12. Related Resource

    Thermo Fisher LTQ VELOS mass spectrometer

13. Service Provided by

    Proteomics Shared Resource Core Laboratory

14. Website(s)

    http://www.ohsu.edu/xd/research/research-cores/proteomics/request-services/index.cfm

15. Website(s)

    http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/index.cfm

16. Related Technique

    Protein identification

17. Related Technique

    Partial protein sequencing