Post-translational modification identification

eagle-i ID

Resource Type

Properties

1. Resource Description

   "This type of analysis is a step beyond simple protein identification. Post-Translational Modifications (PTMs) that result in a simple mass addition to the peptide can be detected using the software present in our lab. A good example of this type of modification is phosphorylation. The mass shift of the potential PTM is entered into the protein identification software, and the software performs multiple searches of potentially modified peptides looking for forms both with and without the PTM. This analysis can take considerably longer because the search time increases geometrically with each potential modification added. Also the algorithms for identifying PTMs are less well developed then those for protein identification, meaning every potential PTM must be verified by hand! This adds considerably to the amount of time it takes to analyze the data, and drives up the overall cost of the experiment. As with protein identification, samples can be submitted as either a liquid solution of the protein(s), or as a band from a gel. Liquid samples may also by lyophilized or taken to dryness before submission."

2. Service Fee URL

   http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/rates.cfm

3. Contact

   David, Larry, Ph.D.

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   Agilent Capillary liquid chromatography system

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   SEQUEST

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   Mascot

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   Scaffold

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   Proteomic Analysis Workflow pipeline

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   Waters nanoACQUITY UPLC chromatography system

10. Service Provided by

    Proteomics Shared Resource Core Laboratory

11. Website(s)

    http://www.ohsu.edu/xd/research/research-cores/proteomics/request-services/index.cfm

12. Website(s)

    http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/index.cfm