Material analysis service
material analysis service
Post-translational modification identification
http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/index.cfm
Website(s)
has URL
has_url
http://www.ohsu.edu/xd/research/research-cores/proteomics/request-services/index.cfm
"This type of analysis is a step beyond simple protein identification. Post-Translational Modifications (PTMs) that result in a simple mass addition to the peptide can be detected using the software present in our lab. A good example of this type of modification is phosphorylation. The mass shift of the potential PTM is entered into the protein identification software, and the software performs multiple searches of potentially modified peptides looking for forms both with and without the PTM.
This analysis can take considerably longer because the search time increases geometrically with each potential modification added. Also the algorithms for identifying PTMs are less well developed then those for protein identification, meaning every potential PTM must be verified by hand! This adds considerably to the amount of time it takes to analyze the data, and drives up the overall cost of the experiment.
As with protein identification, samples can be submitted as either a liquid solution of the protein(s), or as a band from a gel. Liquid samples may also by lyophilized or taken to dryness before submission."
Resource Description
resource_description
http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/rates.cfm
Service Fee URL
Service fee URL
Related Resource
provides access to
provides_access_to
Mascot
Agilent Capillary liquid chromatography system
SEQUEST
Scaffold
Proteomic Analysis Workflow pipeline
Waters nanoACQUITY UPLC chromatography system
Contact
has contact
has_contact
David, Larry, Ph.D.
Service Provided by
service provided by
service_provided_by
Proteomics Shared Resource Core Laboratory
2010-07-30T15:16:48.880-05:00
mhan (Mikyung Han)
2013-03-01T16:45:26.205-06:00
workflow state
Published
shoffmann (Scott Hoffmann)
nvasilevsky (Nicole Vasilevsky)
etl
Service
service
service offering
Analysis service
analysis service
information content entity
information content entity
continuant
entity
generically dependent continuant