eagle-i Oregon Health & Science UniversityOregon Health & Science University
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Label-free relative quantitative analysis

eagle-i ID

http://ohsu.eagle-i.net/i/0000012a-24ff-f265-d994-629180000007

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "Relative Quantitation of the same protein in different samples is usually performed via spectral counting in our laboratory. This type of analysis is simple and easy to perform, as the number of times MS/MS spectra matches to a protein entry in the database has been shown to be roughly proportional to the amount of protein present. Though the analysis of this data is relatively straight forward it requires that more effort is spent on preparing the samples. Errors introduced in the sample preparation phase can result in serious problems with the analysis. Samples for this kind of analysis are usually submitted as either a liquid solution, or lyophilized powder. Single gel bands are not generally compatible with the procedure however, as the counting statistics are affected by the nature of the sample. In this case the abundance difference apparent on gel is a more useful then the spectral counting numbers."
  3. Service Fee URL
    http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/rates.cfm
  4. Contact
    David, Larry, Ph.D.
  5. Related Resource
    Thermo Fisher LTQ spectrometer
  6. Related Resource
    Thermo Fisher LTQ VELOS Pro spectrometer
  7. Related Resource
    Agilent Capillary liquid chromatography system
  8. Related Resource
    SEQUEST
  9. Related Resource
    Mascot
  10. Related Resource
    Scaffold
  11. Related Resource
    Proteomic Analysis Workflow pipeline
  12. Related Resource
    Waters nanoACQUITY UPLC chromatography system
  13. Related Resource
    Thermo Fisher LTQ VELOS mass spectrometer
  14. Service Provided by
    Proteomics Shared Resource Core Laboratory
  15. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/proteomics/request-services/index.cfm
  16. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/proteomics/services-costs/index.cfm
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016