eagle-i Oregon Health & Science UniversityOregon Health & Science University
See it in Search
This page is a preview of the following resource. Continue onto eagle-i search using the button on the right to see the full record.

Nucleic acid sample preparation for sequencing protocol

eagle-i ID

http://ohsu.eagle-i.net/i/0000012a-2501-6065-d994-629180000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    "You can really save some money by doing the cycle sequencing reactions yourself. Note that this does include cleaning up the labeled extension products prior to submitting them for the 3130xl. This mode is appropriate for very experienced clients with proven sample preparation systems and high-throughput needs. D-mode is not for samples that require troubleshooting, additional reagents, special cycling, data processing or analysis: use B-mode for those." See website for details. Protocols specific for: * Single tube samples * Samples in 96 well plate(s)
  2. Uses
    ABI 3130xl genetic analyzer
  3. Used by
    DNA Sequence Analysis Shared Resource
  4. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/dna-analysis/dna-sequencing/sample_preparation.cfm
  5. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/dna-analysis/dna-sequencing/upload/Dmode_PlateProtocol.docx
  6. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/dna-analysis/dna-sequencing/upload/Dmode_TubeProtocol.docx
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016