Nucleic acid fragment analysis

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "* Perform fragment generation for using your favorite protocol. * Clean up fragments to remove unincorporated nucleotides, primers, and salts. * Final sample concentration must be at 200ng in 1uL; If multiplexing, samples must also be at 200ng in 1uL. The maximum volume in each tube or well can ONLY BE 8uL (ex: If using G5, you can use the four dyes available twice, as long as the expected alleles vary in size. This would give a maximum value of 8uL. If you are unsure about how this works or would like to try other combinations please speak with us.)" We currently use: Dye Set: 6-FAM (blue), HEX (green), NED (yellow), ROX (red) Dye Set G5-DS-33: 6-FAM (blue), VIC (green)), NED (yellow), PET (red), LIZ (orange) We use GS500 (-250) LIZ or ROX as the size standard.

3. Service Fee URL

   http://www.ohsu.edu/xd/research/research-cores/dna-analysis/about/fees.cfm

4. Additional Name

   Amplified fragment length polymorphism analysis

5. Additional Name

   Genotyping

6. Additional Name

   Microsatellite marker anlaysis

7. Additional Name

   Single tandem repeat (STR) analysis

8. Contact

   Keller, Thomas J., PhD

9. Related Resource

   ABI 3130xl genetic analyzer

10. Related Resource

    BigDye Terminator Version 3.1 Cycle Sequencing Kit

11. Service Provided by

    DNA Sequence Analysis Shared Resource

12. Website(s)

    http://www.ohsu.edu/xd/research/research-cores/dna-analysis/dna-sequencing/sample_preparation.cfm#CP_JUMP_492703

13. Related Technique

    Nucleic acid fragment analysis

14. Related Technique

    Amplified Fragment Length Polymorphism PCR

15. Related Technique

    Genotyping assay