eagle-i Oregon Health & Science UniversityOregon Health & Science University
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Nucleic acid fragment analysis

eagle-i ID

http://ohsu.eagle-i.net/i/0000012a-2501-7c81-d994-629180000005

Resource Type

  1. Material analysis service

Properties

  1. Fee for service
    Yes
  2. Resource Description
    "* Perform fragment generation for using your favorite protocol. * Clean up fragments to remove unincorporated nucleotides, primers, and salts. * Final sample concentration must be at 200ng in 1uL; If multiplexing, samples must also be at 200ng in 1uL. The maximum volume in each tube or well can ONLY BE 8uL (ex: If using G5, you can use the four dyes available twice, as long as the expected alleles vary in size. This would give a maximum value of 8uL. If you are unsure about how this works or would like to try other combinations please speak with us.)" We currently use: Dye Set: 6-FAM (blue), HEX (green), NED (yellow), ROX (red) Dye Set G5-DS-33: 6-FAM (blue), VIC (green)), NED (yellow), PET (red), LIZ (orange) We use GS500 (-250) LIZ or ROX as the size standard.
  3. Service Fee URL
    http://www.ohsu.edu/xd/research/research-cores/dna-analysis/about/fees.cfm
  4. Additional Name
    Amplified fragment length polymorphism analysis
  5. Additional Name
    Genotyping
  6. Additional Name
    Microsatellite marker anlaysis
  7. Additional Name
    Single tandem repeat (STR) analysis
  8. Contact
    Keller, Thomas J., PhD
  9. Related Resource
    ABI 3130xl genetic analyzer
  10. Related Resource
    BigDye Terminator Version 3.1 Cycle Sequencing Kit
  11. Service Provided by
    DNA Sequence Analysis Shared Resource
  12. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/dna-analysis/dna-sequencing/sample_preparation.cfm#CP_JUMP_492703
  13. Related Technique
    Nucleic acid fragment analysis
  14. Related Technique
    Amplified Fragment Length Polymorphism PCR
  15. Related Technique
    Genotyping assay
 
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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016