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Ovarian X-irradiation (OXI) description protocol

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Resource Type

  1. Protocol


  1. Resource Description
    The X-ray machine utilized was a Phillips 324 kVp, and the beam selected for use employed the ISO HK250 technique, which involves an energy spectrum of 122 keV average and 250 keV maximum. The voltage and current settings used were 250 kV and 12 mA, respectively. The beam filtration that provided this spectrum is made up of 1.60 mm copper, including the 4-mm inherent filtration of the machine. The resulting half-value and quarter- value layers were 2.463 mm and 3.37 mm of copper, respectively. This beam was selected based on its relatively high energy combined with high dose rate, which allows good dose uniformity throughout the full depth of the ovary and a quick (13-min total) exposure time. The additional collimator used for the irradiations provided a tight 13 mm diameter beam for the ovary that was within the lead shield device at a distance of 34 cm (distance from the X-ray machine target). The exposure rate and air-kerma rate measured for this beam are traceable to national standards (NIST).
  2. Contact
    Zelinski, Mary, Ph.D.
  3. Uses
    Rhesus macaque (fertility preservation model)
  4. Uses
    Ovarian radiation
  5. Used by
    Mary Zelinski Laboratory
  6. Author
    Zelinski, Mary, Ph.D.
Provenance Metadata About This Resource Record
  1. workflow state
  2. contributor
    nvasilevsky (Nicole Vasilevsky)
  3. created
  4. creator
  5. modified
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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016