Barklis, Eric, Ph.D.

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1. Resource Description

   "Research in the Barklis lab focuses on the assembly and replication of viruses, such as retroviruses, flaviviruses, and hantaviruses, using molecular genetic, biochemical, and biophysical techniques. Molecular genetic and biochemical approaches have been used, and continue to be employed to investigate viral protein interactions, RNA recognition and encapsidation, and cellular factors involved in virus replication and assembly. To analyze virus particles, proteins, and macromolecular complexes, a variety of biophysical methods also are utilized. For characterization of proteins and small oligomers, we resort to sedimentation, crosslinking, dynamic light scattering, fluorescence polarization, circular dichroism (CD), and nuclear magnetic resonance (NMR) spectroscopy methods. Virions and macromolecular complexes assembled in vitro from viral components are examined by transmission electron microscopy (EM) or atomic force microscopy (AFM). Typically, viruses, or virus assemblies formed in solution or on two-dimensional (2D) membrane sheets, are imaged at high magnification, digitized, and used to generate 7-25 angstrom resolution reconstructions using computer-based image alignment and averaging or electron diffraction approaches. Models and predictions from EM and AFM investigations are tested on virus particles and purified proteins using cell culture, nucleic acid binding, protein binding, mutagenesis, and microscopy techniques."

2. First Name

   Eric

3. Last Name

   Barklis

4. Title

   Director of OHSU Electron Microscropy Core Facility

5. Title

   Professor

6. Website(s)

   http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/molecular-microbiology-and-immunology/research/virology/barklis.cfm?WT_rank=1