eagle-i Oregon Health & Science UniversityOregon Health & Science University
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ES cell culture protocol

eagle-i ID

http://ohsu.eagle-i.net/i/0000012e-edff-165e-4384-aa6580000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    "ES cells must be kept in R1 media with LIF. Media must be changed every day and cells need to be passaged well before reaching confluence. Failure to do so results in differentiation of stem cells and renders the culture unusable."
  2. Uses
    Thermo Scientific Forma series double-stacked CO2 tissue culture incubator
  3. Uses
    Cook Medical benchtop embryo incubator
  4. Uses
    Genevac Bio-Cool programmable cryopreservation freezer
  5. Uses
    Embryonic stem cell culture
  6. Used by
    Transgenic Mouse Model Shared Resource
  7. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/transgenics/protocols.cfm
 
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Provenance Metadata About This Resource Record

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016