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ES cell culture protocol

http://ohsu.eagle-i.net/i/0000012e-edff-165e-4384-aa6580000000

Resource Description

"ES cells must be kept in R1 media with LIF. Media must be changed every day and cells need to be passaged well before reaching confluence. Failure to do so results in differentiation of stem cells and renders the culture unusable."
Uses

Thermo Scientific Forma series double-stacked CO2 tissue culture incubator
Uses

Cook Medical benchtop embryo incubator
Uses

Genevac Bio-Cool programmable cryopreservation freezer
Uses

Embryonic stem cell culture
Used by

Transgenic Mouse Model Shared Resource
Website(s)

http://www.ohsu.edu/xd/research/research-cores/transgenics/protocols.cfm

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