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Affymetrix microarray core labeling methods standard labeling assay

eagle-i ID

http://ohsu.eagle-i.net/i/0000012f-791f-2dd2-f561-6e8a80000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    "This document provides a brief description of methods used in the AMC laboratory for sample labeling and array hybridization and processing during the period of Jan 2002 through June 2004 for GeneChip arrays with feature sizes greater than or equal to 18 micron (e.g, HG-U133A, MOE430A, MG-U74A). Note: for sample inputs of less than 4μg or assays with GeneChip 2.0 all-on-one arrays, the protocols described in this document are not accurate."
  2. Contact
    Harrington, Chris, Ph.D.
  3. Uses
    NanoDrop 8000 spectrophotometer
  4. Uses
    Affymetrix Microarray Suite
  5. Uses
    Illumina BeadArray
  6. Uses
    QuantStudio Real-Time PCR
  7. Uses
    Ion Torrent PGM Sequencer
  8. Uses
    Caliper LabChip GX System
  9. Uses
    RNA quality analysis
  10. Uses
    Affymetrix array
  11. Used by
    Gene Profiling Shared Resource Core Laboratory
  12. Author
    Harrington, Chris, Ph.D.
 
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Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016