RNA/DNA sample quality assessment

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "The concentration of samples submitted to the Affymetrix Microarray Core is measured on a dedicated NanoDrop-1000 or NanoDrop-8000 spectrophotometer that is performance checked using DNA standards prior to collecting sample measurements. Using concentration data provided by the investigator's laboratory, the most accurate method is determined for quantification of the RNA/DNA sample. Currently we routinely use both standard absorbance and fluorometric techniques. The integrity of samples submitted to the Affymetrix Microarray Core is assessed using the Agilent 2100 Bioanalyzer, which uses a chip-based approach to perform fluorescence-based capillary electrophoresis. A concentration measurement is also generated, which is compared against optical density (OD) measurements obtained from the NanoDrop spectrophotometer. The goal of a sample quality assessment is to determine the quantity and quality of RNA/DNA sample material prior to performing the microarray assay." Can also be setup to make use of Nanodrop 3300 fluorospectrometer from the DNA services core.

3. Related Resource

   NanoDrop 8000 spectrophotometer

4. Related Resource

   Caliper LabChip GX System

5. Related Resource

   NanoDrop 1000 spectrophotometer

6. Service Provided by

   Gene Profiling Shared Resource Core Laboratory

7. Website(s)

   http://www.ohsu.edu/xd/research/research-cores/gene-profiling-shared-resource/index.cfm

8. Related Protocol(s)

   0000013d-1ce1-1753-6d01-360380000000

9. Related Technique

   RNA quality analysis

10. Related Technique

    DNA quality analysis