Vasectomy of mice (males 6-8 weeks of age)

eagle-i ID

Resource Type

Properties

1. Resource Description

   "The abdomen is cleaned with 70% ethanol, and a 1.0 cm transverse incision is made in the ventro-distal abdomen. The fat pads overlying the testis and vas deferens are grasped and exteriorized using sterile forceps. Both vas deferentia are then cauterized, and the testes are replaced into the abdominal cavity. The incision is closed as described above. Post-surgical care includes a heating pad or lamp to avoid hypothermia until the mice are recovered from anesthesia. Animals are naturally mated or mated to superovulated females and a minimum of 2 plugged, non-pregnant females indicates a successful vasectomy."

2. Uses

   Small animal surgery

3. Used by

   Transgenic Mouse Model Shared Resource

4. Website(s)

   http://www.ohsu.edu/xd/research/research-cores/transgenics/protocols.cfm