Harvest for blastocyst injection

eagle-i ID

Resource Type

Properties

1. Resource Description

   "3 to 5 week old mice are superovulated and checked for the presence of a copulation plug. After cervical dislocation, fertilized embryos are collected by (e0.5) tearing the ampulla and releasing them into a hyaluronidase/M2 solution for dissociation, (e1.5-e2.5) flushing out via the infundibulum of the oviduct or (e3.5) flushing out of the uterus. Embryos are maintained in gassed KSOM or M16 in a water jacketed, 5% CO2 incubator at 37°C and 95% humidity."

2. Uses

   Kopf vertical pipette puller

3. Uses

   Cook Medical benchtop embryo incubator

4. Uses

   Blastocyst injection

5. Uses

   Small animal surgery

6. Used by

   Transgenic Mouse Model Shared Resource

7. Website(s)

   http://www.ohsu.edu/xd/research/research-cores/transgenics/protocols.cfm