eagle-i Oregon Health & Science UniversityOregon Health & Science University
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Harvest for blastocyst injection

eagle-i ID

http://ohsu.eagle-i.net/i/0000012f-7a43-bfee-f561-6e8a80000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    "3 to 5 week old mice are superovulated and checked for the presence of a copulation plug. After cervical dislocation, fertilized embryos are collected by (e0.5) tearing the ampulla and releasing them into a hyaluronidase/M2 solution for dissociation, (e1.5-e2.5) flushing out via the infundibulum of the oviduct or (e3.5) flushing out of the uterus. Embryos are maintained in gassed KSOM or M16 in a water jacketed, 5% CO2 incubator at 37°C and 95% humidity."
  2. Uses
    Kopf vertical pipette puller
  3. Uses
    Cook Medical benchtop embryo incubator
  4. Uses
    Blastocyst injection
  5. Uses
    Small animal surgery
  6. Used by
    Transgenic Mouse Model Shared Resource
  7. Website(s)
    http://www.ohsu.edu/xd/research/research-cores/transgenics/protocols.cfm
 
RDFRDF
 
Provenance Metadata About This Resource Record

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016