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eagle-i ID


Resource Type

  1. Liquid chromatography mass spectrometry platform


  1. Related Software
  2. Resource Description
    With Shimadzu Prominence XR HPLC. "The AB SCIEX QTRAP® 5500 LC/MS/MS system is the next-generation technology which is designed to excel at metabolite identification, detection and confirmation of low-level pesticides, and protein/peptide quantitation for biomarker verification and validation. Redesigned from the ground up using our new, fast eQTM electronics, but bred from our industry standard triple quadrupole line of mass spectrometers, the QTRAP® 5500 system brings in a new era of performance. The QTRAP® 5500 system also houses the most sensitive ion trap in the world-the all new, patented Linear AcceleratorTM trap along with offering ultra-fast scan speeds, and full MS3 capabilities." "The Applied BioSystems 5500 QTRAP triple quadrupole linear ion trap hybrid mass spectrometerprovides the best quantitative and qualitative performance with UPLC capability available from an in-line Shimadzu Prominence system. It performs all the scan modes of the 4000 Q-TRAP and has a higher scan rate for more sensitive ion trap scan modes including MRM3 quantitation for analytes requiring greater sensitivity with high backgrounds. The system has a TurbroV ionization source with both electrospray and atmospheric pressure chemical ionization probes capable of positive and negative ionization mode. Sample analysis and instrument control will be conducted with Analyst software. The system also has LightSight™ Software that is excellent for finding drug metabolites. The software automatically creates highly optimized methods that improve metabolite detection by constructing compound specific multiple reaction monitoring-information dependent acquisition methods (pMRM-IDA) where the survey scan will provide MS/MS data for potential metabolites. The MS/MS spectra generated will be analyzed for structural elucidation by opening MS/MS spectra in ACD/MS Processor software directly from LightSight™."
  3. Manufacturer
    AB Sciex
  4. Model Number
    QTRAP 5500
  5. Website(s)
  6. Website(s)
  7. Related Technique
    High performance liquid chromatography
  8. Related Technique
    Liquid chromatography-tandem mass spectrometry
  9. Location
    Bioanalytical Shared Resource/Pharmacokinetics Core Laboratory
Provenance Metadata About This Resource Record
Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016