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Nonhuman primate contraceptive model protocol

eagle-i ID

http://ohsu.eagle-i.net/i/0000012f-9e7a-50ca-fb77-395980000000

Resource Type

  1. Protocol

Properties

  1. Resource Description
    Animals were treated in accord with the NIH Guide for the Care and Use of Laboratory Animals and under research protocols reviewed and approved by the institutional animal care and use committee of the ONPRC. Anesthetized animals underwent a pre-screening laparoscopy to evaluate ovarian ligaments length. Only those with long ligaments, which were necessary to undergo ovarian manipulations with minimal damage to ovarian circulation, were included in the experiments. Each animal was placed horizontally on a stainless steel table, with a hydraulic lift to facilitate positioning. Stationary laser beams were utilized to position the ovaries at a reproducible point in space associated with an exact calibrated position. Ovaries were positioned carefully outside the ventral surface either with a hemostat attached to the ovarian ligament and surrounded by an additional lead collar to protect the rest of the body, or were placed within a shield that tightly collimated the radiation beam down to 13 mm diameter (the ovary was positioned within a small lead cup at the end of the collimator). Ovaries were covered with sterile gauze sponges soaked in warm sterile saline. Irradiation commenced for a pre-determined duration of 13 min to generate 1,500 rads of absorbed dose (see below). The female was then removed from the irradiation table, sutured and returned to her cage. Animals assigned to sham-irradiation underwent surgical manipulations and procedures (i.e., externalization of the ovary for 20 min) without OXI.
  2. Uses
    Cynomolgus macaque
  3. Uses
    Rhesus macaque (contraception model)
  4. Used by
    Mary Zelinski Laboratory
  5. Author
    Zelinski, Mary, Ph.D.
 
RDFRDF
 
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