"The SK3T mutant allele has a tetracycline-based genetic switch inserted into the 5' UTR of the Kcnn3 (potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3; also called SK3) locus, just upstream of the translation initiation site. This genetic switch harbors both the tetracycline-controlled transactivator protein (tTA) as well as the tetracycline operator (tetO; also called tetracycline-responsive element (TRE) or tet-operator); allowing transcription of the downstream Kcnn3 locus to be blocked by administration of tetracycline (or its analog doxycycline (dox)). Homozygotes exhibit three-fold overexpression of SK3 before, and no SK3 expression during, doxycycline administration."