Arginase-YFP

eagle-i ID

Resource Type

Properties

1. Exchange facilitator

   <a href=http://jaxmice.jax.org/strain/015857.html target=_window><img src=http://eagle-i.net/images/jacksonlabs-button-large.png alt='Order from Jackson Laboratory'></a>

2. Resource Description

   "A targeting vector was designed to insert an internal ribosome entry site (IRES)-enhanced yellow fluorescent protein (eYFP) fusion protein, downstream of the endogenous stop codon of the arginase (Arg1) gene. These homozygous YARG mice are viable, fertile, and normal in size. Located in the cytoplasm of the liver and specifically in macrophages, arginase I converts L-arginine into L-ornithine and urea as the final step in the urea cycle. L-arginine can also be catabolized into nitric oxide (NO), a secreted free radical which causes tissues damage and is toxic to bacteria. Arginase I suppresses NO production by decreasing the amount of L-arginine available to the nitric oxide synthase (NOS) enzyme for conversion into NO and other reactive oxygen species (ROS). Nine days after infection with the helminth, Nippostrongylus brasiliensis, YARG mice exhibit accumulation of arginase I-expressing macrophages in the lung and peritoneum. As soon as two days after inoculation with chitin, a polysaccharide found in the exoskeleton of some invertebrates, these macrophages were also present in the lung and peritoneum. These mice may be useful for visualization of arginase-containing cells and their response to infection."

3. Additional Name

   B6.129S4-Arg1tm1Lky/J

4. Additional Name

   YARG

5. Inventory Number

   Jax Stock Number: 015857

6. Related Disease

   cancer

7. Parental Strain Name

   129S4/SvJae-Tg(Prm-cre)70Og

8. Genetic Alteration(s)

   Arg1[tm1Lky]

9. Phenotype Findings

   Normal phenotype

10. Location

    Lisa Coussens Laboratory