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pCMV-Tag1-Fxna

eagle-i ID

http://ohsu.eagle-i.net/i/0000013f-7775-0441-f351-2c0e80000000

Resource Type

  1. Plasmid

Properties

  1. Construct Insert
    Fxna
  2. Resource Description
    "The coding region of Fxna mRNA was cloned using the FailSafe PCR System Kit (Epicentre Biotechnologies, Madison, WI) and primers (forward, 5-TATAGATCTTGGAGTGGAGCTCGGAAGT-3; reverse, 5-TAT- AGATCTTTAAAACACAAAGAGACTATAGGTGG-3), containing a BglII site at their 5􏰂 ends (underlined). The PCR product was cloned into the pGEM-T vector (Promega, Madison, WI) and sequenced from both ends, before cloning into the BglII site of the expression vector pCMV-Tag1 (Stratagene, San Diego, CA)."
  3. Construct Backbone
    pCMV-Tag1
  4. Related Publication or Documentation
    Fxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis
  5. Location
    Sergio Ojeda Laboratory
 
RDFRDF
 
Provenance Metadata About This Resource Record

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