pCMV-Tag1-Fxna

eagle-i ID

Resource Type

Properties

1. Construct Insert

   Fxna

2. Resource Description

   "The coding region of Fxna mRNA was cloned using the FailSafe PCR System Kit (Epicentre Biotechnologies, Madison, WI) and primers (forward, 5-TATAGATCTTGGAGTGGAGCTCGGAAGT-3; reverse, 5-TAT- AGATCTTTAAAACACAAAGAGACTATAGGTGG-3), containing a BglII site at their 5􏰂 ends (underlined). The PCR product was cloned into the pGEM-T vector (Promega, Madison, WI) and sequenced from both ends, before cloning into the BglII site of the expression vector pCMV-Tag1 (Stratagene, San Diego, CA)."

3. Construct Backbone

   pCMV-Tag1

4. Related Publication or Documentation

   Fxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis

5. Location

   Sergio Ojeda Laboratory