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pcDNA-Zeo-Fxna-FLAG plasmid

eagle-i ID


Resource Type

  1. Plasmid


  1. Construct Insert
  2. Resource Description
    "A tagged Fxna construct was generated by PCR-amplifying the Fxna coding region from ovarian RNA with a sense primer (5􏰂- GGATCCGCTGCCGCCATGGAGTGG-3􏰂) and an antisense primer (5􏰂- GATATCATATTACTTGTCGTCATCGTCTTTGTAGTCA A ACACA A AGA- GACTATA-3􏰂) that contains a FLAG epitope-coding sequence (in italics); BamH1 and EcoRV sequences added to the sense and antisense primers, respectively, are underlined. The resulting construct was ligated into pcDNA-Zeo (Invitrogen)."
  3. Construct Backbone
  4. Related Publication or Documentation
    Fxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis
  5. Location
    Sergio Ojeda Laboratory
Provenance Metadata About This Resource Record
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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016