RNA interference plasmid
pCMV-Tag1-Fxna siRNA
Construct Insert
Construct insert
has part construct insert
construct insert
has_part_construct_insert
Fxna mRNA carrying silent point mutations of the siRNA target region.
construct insert
Fxna mRNA carrying silent point mutations of the siRNA target region.
Rattus norvegicus
http://www.ncbi.nlm.nih.gov/gene/373544
5-AACAGCCTCCACAGAATaTCt
Ermp1
Fxna
processed material
processed material
material_entity
independent_continuant
continuant
entity
"Expression vector (pCMV-Tag1, Stratagene) encoding Fxna mRNA carrying silent point mutations of the siRNA target region. The third base of each of two codons in this region was mutated (5-AACAGCCTCCACAGAATaTCt), using the QuickChange XL Site-Directed Mutagenesis Kit (Stratagene)."
Resource Description
resource_description
pCMV-Tag1
Construct Backbone
construct backbone
construct_backbone
Related Publication or Documentation
has documentation
has_documentation
Fxna, a novel gene differentially expressed in the rat ovary at the time of folliculogenesis, is required for normal ovarian histogenesis
Location
located_in
Sergio Ojeda Laboratory
located in
2013-06-24T13:52:41.648-05:00
nvasilevsky (Nicole Vasilevsky)
2013-06-24T13:52:48.502-05:00
workflow state
Published
nvasilevsky (Nicole Vasilevsky)
Plasmid
recombinant plasmid
recombinant plasmid
Construct
construct
construct
Reagent
reagent
reagent
Gene knockdown reagent
gene knockdown reagent
RNA interference reagent
processed material
processed material
material_entity
independent_continuant
continuant
entity