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Bioanalytical Shared Resource/Pharmacokinetics Core Laboratory

Director: Koop, Dennis, Ph.D.

Summary:

This resource is available at OHSU for the analysis of small molecules such as drugs and drug metabolites, neurotransmitters, fatty acids and biologically active metabolites of fatty acids, steroids, and natural products . The BSR provides open access to a laboratory where users can prepare and analyze their own samples by HPLC, GC/MS or LC/MS on equipment maintained by Core personnel. In addition, samples can be submitted for complete analysis by Core staff and the Director and Associate Director are available for assay development assistance.

Affiliations:

People:

Resources:

Instruments

  • AB SCIEX QTRAP 4000 LC/MS/MS System ( Liquid chromatography mass spectrometry platform )

    With Shimadzu Prominence XR HPLC and UV/VIS Detector "The Applied BioSystems 4000 QTRAP triple-quadrupole, linear ion trap hybrid MS provides maximum quantitative and qualitative performance with UPLC capability available from an in-line Shimadzu Prominence system. A second set of Shimadzu pumps can be used for automated on line sample extraction using solid phase extraction. The system has a TurboV ionization source with an electrospray or atmospheric pressure chemical ionization probe capable of positive and negative ionization mode, allowing detection of a wide range of molecules. A photodiode array detector is also available prior to mass spectral analysis. Sample analysis and instrument control will be conducted with Analyst software. The combination of true triple-quadrupole and sensitive ion trap scan modes in a single LC/MS/MS run by the QTRAP allows for results that would otherwise require multiple analyses on multiple MS platforms. The system also has LightSight™ Software that is excellent for finding drug metabolites. The software automatically creates highly optimized methods that improve metabolite detection by constructing compound specific multiple reaction monitoring-information dependent acquisition methods (pMRM-IDA) where the survey scan will provide MS/MS data for potential metabolites. The MS/MS spectra generated will be analyzed for structural elucidation by opening MS/MS spectra in ACD/MS Processor software directly from LightSight™."

  • AB SCIEX QTRAP 5500 LC/MS/MS System ( Liquid chromatography mass spectrometry platform )

    With Shimadzu Prominence XR HPLC.

    "The AB SCIEX QTRAP® 5500 LC/MS/MS system is the next-generation technology which is designed to excel at metabolite identification, detection and confirmation of low-level pesticides, and protein/peptide quantitation for biomarker verification and validation. Redesigned from the ground up using our new, fast eQTM electronics, but bred from our industry standard triple quadrupole line of mass spectrometers, the QTRAP® 5500 system brings in a new era of performance.

    The QTRAP® 5500 system also houses the most sensitive ion trap in the world-the all new, patented Linear AcceleratorTM trap along with offering ultra-fast scan speeds, and full MS3 capabilities."

    "The Applied BioSystems 5500 QTRAP triple quadrupole linear ion trap hybrid mass spectrometerprovides the best quantitative and qualitative performance with UPLC capability available from an in-line Shimadzu Prominence system. It performs all the scan modes of the 4000 Q-TRAP and has a higher scan rate for more sensitive ion trap scan modes including MRM3 quantitation for analytes requiring greater sensitivity with high backgrounds. The system has a TurbroV ionization source with both electrospray and atmospheric pressure chemical ionization probes capable of positive and negative ionization mode. Sample analysis and instrument control will be conducted with Analyst software. The system also has LightSight™ Software that is excellent for finding drug metabolites. The software automatically creates highly optimized methods that improve metabolite detection by constructing compound specific multiple reaction monitoring-information dependent acquisition methods (pMRM-IDA) where the survey scan will provide MS/MS data for potential metabolites. The MS/MS spectra generated will be analyzed for structural elucidation by opening MS/MS spectra in ACD/MS Processor software directly from LightSight™."

  • ABI 4000 Q TRAP mass spectrometer ( Liquid chromatography mass spectrometry platform )

    "Multiple reaction monitoring (MRM) experiments are carried out with this instrument which contains, an advanced hybrid triple quadrupole/linear ion trap. This instrument uses MDS SCIEX Linear Ion Trap (LIT) technology, which enables excellent quantitative and qualitative performance within a single system. Use of a quadrupole as a linear ion trap significantly enhances ion trap performance by increasing ion capacity, improving injection and trapping efficiencies, and increasing duty cycle. This system provides true triple quadrupole MRM at the highest level of sensitivity, as well as extended dynamic range, ensuring superior quantitation performance for peptides. The Q TRAP can be configured with either the TurboIonSpray source or the MicroIon source, mounted on the MDS Sciex nanosource."

  • Thermo TSQ Quantum LC/MS/MS System ( Mass spectrometer )

    "The TSQ can be used for reproducible and selective quantification by LC/MS or MS/MS and can also perform sensitive precursor ion scanning experiments to generate parent ion spectra for a particular fragment or neutral loss. The mass spectrometer has an electrospray or atmospheric pressure chemical ionization probe, each capable of positive and negative ionization modes, allowing for the analysis of a wide range of molecules. In addition the instrument also has an atmospheric pressure photo-ionization probe for molecules difficult or impossible to detect using other atmospheric pressure ionization techniques. The mass spectrometer has a ultra high pressure Accela HPLC system and autosampler that allows the use of sub 2μ HPLC columns. Instrument control and data analysis is afforded by Xcalibur software."

  • Trace DSQ GC/MS System ( Tandem mass spectrometer )

    "This is a highly sensitive automated GC/MS instrument with an autosampler and split/spiltless injector and used for molecules requiring a gas chromatographic interface for separation. It can be used for electron impact or chemical ionization in positive and negative modes. Instrument control and data analysis is afforded by Xcalibur software."

Services

Software

  • Agilent ChemStation ( Software )

    "Agilent ChemStation is the industry leading chromatography data system for Agilent instrumentation, handling the widest variety of separation techniques such as GC, LC, LC/MS, CE and CE/MS. It is a scalable data system ideally suited for applications in all industries ranging from early product development to quality control. Extensive customization capabilities as well as configurable regulatory compliance provide the flexibility to support different workflows. Sophisticated level-5 control and monitoring of LAN-based instruments ensures fast and flexible data acquisition, which is complemented by advanced data analysis and reporting capabilities for highest productivity." The software performs mass spectrometry instrument control and LC-MS data analysis.

  • Analyst ( Software )

    Mass spectrometry instrument control software.

  • LightSight ( Software )

    "LightSight® software lets you efficiently identify and confirm metabolites from data acquired on AB SCIEX triple quadrupole and hybrid linear ion trap mass spectrometers."

  • Xcalibur ( Software )

    A "Windows-based data system that provides instrument control and data analysis for the entire family of Thermo Scientific mass spectrometers and related instruments."


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Last updated: 2012-08-15T15:43:39.910-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016