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Histopathology Shared Resource

Director: Thomas, George, M.D.

Summary:

The Histopathology Shared Resource is a College of American Pathologists (CAP) certified and Clinical Laboratory Improvement Amendments (CLIA) accredited core facility that provides investigators with tissue-based services fundamental to the progress of basic, translational and clinical research.

The mission the Histopathology Shared Resource (HSR) is to:

1. Provide investigators with histology services critical for the progress of biomedical research involving studies on research animal and human tissues These services include cryostat sectioning, paraffin- embedding, cell pellet and cytospin preparation, microtome-sectioning, histochemical staining and immunohistochemistry.

2. Provide investigators with consultation and education in regard to best use of histopathology services research protocols, safety issues, and IRB application.

Affiliations:

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Instruments

Services

  • Histology tissue and staining services ( Material processing service )

    "This core facility is equipped and staffed to provide a full array of histology services including Tissue processing and paraffin embedding, Sectioning (cryostat or paraffin-embedded) to specified thickness and Histochemical stains, such as:

    Hematoxylin and eosin (H&E)
    Trichrome
    Elastin, Reticulin
    Giemsa
    Luxol fast blue
    Iron
    Bacterial and fungal"

  • Immunohistochemistry staining service ( Material processing service )

    "The Histopathology Shared Resource provides Immuhistochemistry services which include:

    Testing and titering new antibodies, both monoclonal and polyclonal. The latter should be either a purified IgG fraction or affinity-purified. (Straight serum is not suitable for immunostaining).
    Optimization of staining to enhance detection and reduce background.
    Epitope retrieval techniques (pre-treatment with heat and/or proteases)
    Specialized secondary detection reagents
    Avidin/biotin blocking steps
    Double- and triple-antibody stains using multiple chromagens."


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Last updated: 2013-11-05T13:22:28.171-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016