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Jon's Primate Reproductive System Laboratory

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Instruments

Protocols

  • Controlled ovarian stimulation protocol ( Protocol )

    Controlled ovarian stimulation (COS) protocols were developed in rhesus monkeys that are similar to those used in clinical in vitro fertilization practice. COS protocols are used to study periovulatory events in the primate follicle, as well as to investigate oocyte maturation, embryo development, and the generation of embryonic stem cells.

    Referenced in: Dynamics of periovulatory steroidogenesis in the rhesus monkey follicle after ovarian stimulation. PMID: 10221689

  • Controlled ovulation protocol ( Protocol )

    The controlled ovulation (COv) protocol allows for a physiological model to investigate the ovulatory, luteinizing follicle in primates. To overcome the major limitation of studies in spontaneous cycles, i.e., the difficulty in determining when the midcycle LH surge begins and removing follicular tissues at precise intervals thereafter, the COv protocol in adult female rhesus monkeys permits precise timing for the induction of periovulatory events.

    Publication:
    Controlled ovulation of the dominant follicle: a critical role for LH in the late follicular phase of the menstrual cycle.
    PMID: 14585870

  • Intrafollicular injection protocol ( Protocol )

    The intrafollicular injection (IFI) technique was developed as means to administer substances into the preovulatory follicle of monkeys during the menstrual cycle. This procedure requires only small amounts of bioactive material, and allows direct access of the injected substances to the oocyte and granulosa cells. In addition, injection by itself (plus the surgery or ovarian manipulation) does not interfere with timely ovulation and subsequent luteal development.

    Publication:
    Local delivery of angiopoietin-2 into the preovulatory follicle terminates the menstrual cycle in rhesus monkeys.
    PMID: 15703373

  • Natural menstrual cycle monitoring protocol ( Protocol )

    Circulating levels of estradiol are monitored beginning several days after menses to determine the timing of the midcycle release of luteinizing hormone that results in ovulation. This allows for the precise staging of the corpus luteum through its period of development, peak function, and regression.

    Publication:
    Prostaglandin synthesis, metabolism, and signaling potential in the rhesus macaque corpus luteum throughout the luteal phase of the menstrual cycle.
    PMID: 18635657

  • Primate granulosa cell isolation protocol ( Protocol )

    This protocol allows for the collection and isolation of granulosa cells from the rhesus macaque ovulatory follicle and their use in subsequent in vitro studies.

  • Primate luteal cell suspension protocol ( Protocol )

    This protocol allow for the dispersion of the primate corpus luteum such that cellular processes may be studied and/or manipulated in vitro.

  • Reagent delivery by osmotic minipump to the primate corpus luteum ( Protocol )

    The following protocol was established to deliver test reagents to the primate corpus luteum, using osmotic minipumps. This allows for local delivery of materials to the rhesus macaque corpus luteum over a wide range of times and at different stages of the luteal phase.

  • Simulated early pregnancy protocol ( Protocol )

    The simulated early pregnancy protocol provides a model system wherein the function of the rhesus macaque corpus luteum is maintained in the absence of implantation through the administration of escalating doses of the pregnancy hormone chorionic gonadotropin. This allows for the study of the cellular events that are involved in determining luteal lifespan and function.


    Publication:
    Changes in available gonadotropin receptors in the corpus luteum of the rhesus monkey during simulated early pregnancy.
    PMID: 6329648

Reagents


Last updated: 2011-04-19T17:45:44.161-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016