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Molecular Virology Support Core

Summary:

The overall goal of the Molecular Virology Support Core (MVSC) is to advance the efforts of the Oregon National Primate Research Center (ONPRC), the Vaccine and Gene Therapy Institute (VGTI), and collaborative research programs focused on nonhuman primate (NHP) infectious disease models and those using viral vectors as tools to modify genes or deliver macromolecules through provision of expertise, reagents, standardized assays, and training in the use of infectious viral agents in their research programs.

Affiliations:

People:

Resources:

Instruments

  • ABI 7500 quantitative PCR machine ( Real-time PCR machine )

    "The Applied Biosystems 7500 Fast Real-Time PCR System offers maximum performance in the minimum time. Fully optimized for Fast cycling, the 7500 Fast delivers high-quality results in as little as 30 minutes.
    - Specially designed Fast block ensures thermal uniformity at top speeds
    - Faster ramp rates enable rapid results without compromising extension times or assay quality
    - Fast optical plates ensure excellent precision in 10-30 uL reaction volumes
    - Use TaqMan Fast Universal PCR Master Mix to run multiple Fast assays on one plate
    - Finish more runs per day with the excellent performance equivalent to that offered by the 7500 System"

  • ABI GeneAmp 9700 96-well PCR system ( PCR instrument )

    "GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications."

  • Beckman Coulter Allegra 6R centrifuge ( Refrigerated centrifuge )

    "The Allegra 6 Series is designed for high-volume labs requiring a simple, durable solution above all else. Save time with the basic, streamlined user interface that is easy to use and quick to program. Available in refrigerated and ambient benchtop and refrigerated knee-well models."

  • Beckman Coulter L7-80 ultracentrifuge ( Ultracentrifuge )

    This is a floor unit with offering a maximum 80000 rpm.

  • Beckman Coulter Microfuge 22R microcentrifuge ( Microcentrifuge )

    Bench-top unit with a brushless induction drive has a 24-place fixed angle rotor able to hold 1.5–2.0mL tubes.

  • Biotek ELx405UCWS ( Microplate washer )

    "- Accommodates 384- and 96-well microplates
    - Polystyrene and magnetic bead assays along with standard ELISAs
    - Patented Dual-Action™ manifold on the ELx405™ HT and ELx405™ Select provides independent control of the aspirate and dispense manifolds for precise overfill washing and overflow protection
    - Internal positive displacement pump eliminates the use of troublesome pressure delivery systems and allows the use of any size dispense reservoir
    - Built-in priming trough allows automatic manifold priming and rinsing during both operation and maintenance modes and eliminates the need for blank plates when used in automated robotic systems
    - Fully variable dispense volumes, flow, and aspiration rates provide total control of the washing cycles from a gentle cell-washing application to vigorous wash for high-binding assays
    - Built-in fluid flow, fluid detection, and vacuum sensing provide complete protection for unattended operation
    - Optional external valve module connects four reagent bottles with automatic switching between reagents
    - BioStack compatible for automated washing of up to 50 standard height microplates"

  • Biotek Synergy Mx mono-based microplate reader ( Microplate reader )

    Modules include: fluorescence, absorbance, and luminescence.

  • CBS Isothermal V1500-AB dewar ( Liquid nitrogen dewar )

    "- Liquid Nitrogen Capacity (Liters): 30
    - Static Evaporation Rate (Liters / Day): 5
    - Static Holding Time (Days): 6
    - Weight Empty (lbs / kg): 327 / 148
    - Weight Full (lbs / kg): 383 / 174"

  • Eppendorf AG microcentrifuge ( Microcentrifuge )

  • Leica inverted microscope with CoolLED pE-100 ( Inverted microscope )

    This setup is used for fluorescent microscopy (470 nm excitation).

  • MagNA Lyser tissue homogenizer ( Homogenizer )

    "The MagNA Lyser Instrument is used for automated, efficient homogenization of tissue samples for different extraction applications. This benchtop instrument, together with the MagNA Pure LC Instrument for extraction of nucleic acids, the LC Carousel Centrifuge 2.0, and the LightCycler® Instruments for real-time PCR, perfectly integrates into your laboratory workflow."

  • MagNA Pure Compact for nucleic acid isolation ( Nucleic acid extraction/purification instrument )

    "The MagNA Pure Compact System is the automated benchtop solution for nucleic acid purification. With its small instrument size, extensive integrated features and a sample throughput of one to eight samples per run, the system meets the demanding nucleic acid isolation needs of research laboratories with low to medium sample throughput. The high-quality nucleic acids isolated with the MagNA Pure Compact System are suitable for a broad range of applications in nucleic acid research, including gene expression analysis (using PCR and RT-PCR on the LightCycler® System, or on standard block cyclers), microarray analysis, genotyping reactions using the LightTyper Instrument, and many other downstream technologies."

  • New Brunswick Scientific Excella NB24 incubator shaker ( Incubator shaker )

    "New Brunswick Scientific’s Excella line of biological
    shakers provides advanced features and excellent
    performance at an affordable price. All eight
    temperature-controlled and open-air Excella models
    feature heavy-duty drives, providing long life and quiet
    operation under moderate loads and at speeds up to
    400 rpm."

  • NuAire Labgard ES safety cabinet ( Biological safety cabinet )

    Cabinet meets Class II and Type A2 standards.

  • PCR hood ( Nucleotide-free workbench )

  • Sanyo -80°C Freezer ( Freezer )

  • SterilGard bio-safety cabinet ( Biological safety cabinet )

    Meets/exceeds Class II and Type A/BS specifications.

  • Sterisonic GxP Cell Culture CO2 Incubator with SafeCell UV ( CO2 incubator )

    "The SANYO Sterisonic™ GxP incubator is designed for a wide array of demanding and highly regulated applications in the biomedical, pharmaceutical, medical research and clinical laboratory. Representing years of research, development and component testing, the Sterisonic™ GxP incorporates a collective of mutually functional systems and design attributes to offer a holistic solution to cell culture protocols, from the most sophisticated to more familiar and conventional processes."

  • VirTis VirSonic ultrasonic cell disruptor ( Sonicator )

Services

Software

  • Gen5 Secure ( Software )

    This software is used to control and analyze data from the Biotek Synergy Mx Mono-based microplate reader.


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Last updated: 2012-12-14T16:34:57.334-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016