eagle-i Oregon Health and Science UniversityOregon Health and Science University
See it in Search

Flow Cytometry Shared Resource

Director: Streeter, Philip R., Ph.D.

Summary:

The OHSU Flow Cytometry Shared Resource (FCSR) has operated as a core resource for OHSU Knight Cancer Institute members since 1996 and provides advanced flow cytometry instrumentation, technical expertise and technical services. The FCSR also provides training in data interpretation, experiment design and routine operation to researchers, offering an additional cost-saving option of doing some of the work themselves. Finally, this resource saves valuable investigator time by analyzing specimens and preparing them, if needed.

People:

Resources:

Instruments

  • BD Canto II® flow cytometer ( Flow cytometer analyzer )

    "5-color system with a UV laser for Hoechst/DAPI viability or cell cycle analysis."

  • BD FACSCalibur flow cytometer ( Flow cytometer analyzer )

    "The BD FACSCalibur™ platform offers a unique modular approach to flow cytometry and allows users to perform both cell analysis and cell sorting in an innovative single benchtop system. Designed with multicolor application support in mind, the BD FACSCalibur flow cytometer provides flexibility to support a wide variety of research and clinical applications."

  • BD Fortessa ( Flow cytometer analyzer )

    "Four lasers and 18 parameters expands the amount of data collected from a single tube. This analyzer is optimal for experiments with limited cell numbers."

  • BD InFlux flow cytometer sorter ( Flow cytometer sorter )

    "The BD Influx system can sort at high speeds to support a variety of applications including quick enrichment of rare event populations. In addition, the Computerized Cell Deposition Unit (CCDU) built into the BD Influx allows for automated single cell deposition for cloning or single-cell PCR. The unit is fast and configurable and can be adapted to user-defined collection devices." "Can sort up to eleven fluorescent probes rapidly to purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously."

  • BD LSR II flow cytometer ( Flow cytometer analyzer )

  • BD Turbo DiVa Vantage® cell sorter ( Flow cytometer sorter )

    "Can sort up to eight different fluorescent probes at up to 20,000 cells/second and purify populations that are less than 1% of the original mixture to >98% purity. Able to sort into plates or up to four populations simultaneously."

  • Beckman Coulter Vi-Cell® ( Automatic cytometer )

    "Fast and cost-effective cell counter adds trypan to samples and generates images and data representing cell size distribution, concentration and viability."

  • DVS Sciences CyTOF mass cytometer ( Mass spectrometer )

    "Mass Cytometer capable of highly multiplexed, single cell analysis of up to 33+ metal labeled antibodies, without the need for compensation."

  • Luminex 200® ( Bead-based multiplex )

    "For genomic and proteomic assays including protein expression, cell signaling, genotyping and metabolic markers. Multiplex up to 100 analytes in one microplate well."

  • Miltenyi AutoMACS Pro Separator ( Cell separator )

    "Magnetic bead separation allows for cell enrichment or pre-sorting prior to InFlux or Vantage use."

  • Miltenyi MACSQuant Analyzer ( Flow cytometer analyzer )

    "Three-laser system allowing for up to seven-color analysis from tubes or 96-well plates, with absolute cell counts by volume."

Protocols

Services

  • Analytical flow cytometry service ( Material analysis service )

    * Quantitative measurement of fluorescent reporters to assess the distribution of specific molecules within cell populations
    * Analysis of multiple characteristics such as relative cell size, antibody binding to cell surface or intracellular proteins, DNA and RNA content, and fluorescent protein expression.
    * Functional assays can measure apoptosis, enzyme activity and calcium flux
    * After acquisition of data, you will receive files and may make printouts if desired.

  • Cell sorting service ( Material production service )

  • Flow cytometry consultation service ( Support service )

    The Flow Cytometry Shared Resource offers flow cytometry-related technical expertise for standard and custom research applications.

  • Flow cytometry data analysis service ( Data analysis service )

  • Flow cytometry instrument training ( Training service )

    "* Quantitative measurement of fluorescent reporters to assess the distribution of specific molecules within cell populations
    * Analysis of multiple characteristics such as relative cell size, antibody binding to cell surface or intracellular proteins, DNA and RNA content, and fluorescent protein expression.
    * Functional assays can measure apoptosis, enzyme activity and calcium flux"

  • Grant preparation support service ( Support service )

    This lab is able to assist with preparation for grant applications, relating to cell cycle analysis, cell sorting and other flow cytometry analyses.

Software

  • BD FACSDiva ( Software )

    "BD FACSDiva™ software is a collection of rich tools for flow cytometer and application setup, data acquisition, and analysis that help streamline flow cytometry workflow for today's busy laboratory. BD FACSDiva software offers built-in features for consistent and maintainable daily cytometer setup that supports reproducible data acquisition and reliable results."

  • FCS Express ( Software )

    This software is used for flow cytometry data analysis and "follows the standards set by common Microsoft Office™ Products, particularly Powerpoint™."

  • FlowJo ( Software )

    Flow cytometry data and statistical analysis software. The laboratory has access to a site license. Contact the laboratory for more information.

  • ModFit LT ( Software )

    "This software provides open-ended modeling of flow cytometry histograms. Models for use with cell-tracking dye studies and synchronized cell lines are built right into the software."

  • xPONENT® 3.1 Software ( Software )

    For use with the Luminex 200 System.


Web Links:

Last updated: 2013-02-26T11:37:45.714-06:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016