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Michael Liskay Laboratory

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Organisms and Viruses

  • AS4 ( Saccharomyces cerevisiae )

    MATalpha trp1 arg4 tyr7 ade6 ura3

  • Mlh1-Deficient Mice ( Mus musculus )

    MLH1 is one of the genes involved in DNA repair during cell division. MLH1 knock-out mice and cell lines provide a novel model to study cancer and could potentially aid in the development of new therapeutics for oncology. Licensing and collaborative research opportunities exist with respect to these biological materials.

    In humans, most germline mutation carriers are heterozygotes (+/-) carrying recessive MLH1 mutations in all tissues. When the other MLH1 gene is inactivated in a particular cell, in the lining of the colon for example, the mutation rate in that cell and its progenitors increases dramatically, mutations accumulate and tumors can arise.

  • NEY1084 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 ogg1::TRP1

  • NEY1086 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 ogg1::TRP1

  • NEY1088 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 pms1::URA3 ogg1::TRP1

  • NEY1090 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 pms1::URA3 ogg1::TRP1

  • NEY1092 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 pms1-G882E ogg1::TRP1

  • NEY1094 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 pms1-H888R ogg1::TRP1

  • NEY1096 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 pms1-G882E ogg1::TRP1

  • NEY1098 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 pms1-H888R ogg1::TRP1

  • NEY1100 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1::TRP1 msh2::LEU2

  • NEY1106 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 msh2::LEU2

  • NEY1110 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-G882E msh2::LEU2

  • NEY1126 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-H888R msh2::LEU2

  • NEY186 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 hom3-10 pms1::TRP1

  • NEY190 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 hom3-10.

    Wild-type endogenous PMS1 locus.

  • NEY398 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 hom3-10 pms1-G882E

  • NEY402 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5 hom3-10 pms1-H888R

  • NEY442 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his1-7 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1::TRP1

  • NEY506 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his1-7 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-H888R.

  • NEY510 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his1-7 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-G882E

  • NEY524 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his1-7 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5; HIS3 his1-7 derivative of NEY190

  • NEY570 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5; HIS3 his7-2 derivative of NEY190

  • NEY574 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-H888R

  • NEY578 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-G882E

  • NEY630 ( Saccharomyces cerevisiae )

    MATalpha trp1 arg4 tyr7 ade6 ura3 pms1::URA3

  • NEY662 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1::TRP1

  • NEY669 ( Saccharomyces cerevisiae )

    MATalpha trp1 arg4 tyr7 ade6 ura3 pms1-H888R

  • NEY673 ( Saccharomyces cerevisiae )

    MATalpha trp1 arg4 tyr7 ade6 ura3 pms1-G882E

  • NEY702 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 pms1::URA3

  • NEY706 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 pms1::URA3

  • NEY714 ( Saccharomyces cerevisiae )

    MATa leu2 ade6 ura3 his4-713 pms1::URA3

  • NEY726 ( Saccharomyces cerevisiae )

    MATa leu2 ade6 ura3 his4-713 pms1-H888R

  • NEY738 ( Saccharomyces cerevisiae )

    MATa leu2 ade6 ura3 his4-713 pms1-G882E

  • NEY742 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 pms1-G882E

  • NEY744 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52 pms1-H888R

  • NEY747 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 pms1-G882E

  • NEY749 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52 pms1-H888R

  • NEY815 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pol2-C1089Y

  • NEY819 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1::TRP1 pol2-C1089Y

  • NEY823 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-G882E pol2-C1089Y

  • NEY827 ( Saccharomyces cerevisiae )

    MATa ade2-1 CAN1 his7-2 hom3-10 leu2-3,112 trp1-1 ura3-1 RAD5 pms1-H888R pol2-C1089Y

  • PD24 ( Saccharomyces cerevisiae )

    MATa leu2 ade6 ura3 his4-713.

    Wild-type strain.

  • Pms2 cre Apc fx conditional knockout mice ( Mus musculus )

    DNA mismatch repair (MMR) leads to an increased mutation frequency and a predisposition to neoplasia. Knockout mice deficient in the MMR proteins PMS2 crossed with mutation detection reporter (supF) transgenic mice have been used to facilitate a comparison of the changes in mutation frequency and spectra. PMS2 -deficient mice accumulate transition mutations as the predominant mutation.

    Both male and female mice are available.

  • Pms2 cre beta-Galactosidase conditional knockout mice ( Mus musculus )

  • Pms2 cre GFP knockout mice ( Mus musculus )

  • Pms2 Knockout mouse ( Mus musculus )

    Mutations in the DNA mismatch repair pathway have been shown to be involved in the development of certain cancers. PMS2 is one of the genes involved in the DNA mismatch/repair pathway. PMS2 knock-out mice and cell lines provide a novel model to study cancer and could potentially aid in the development of new therapeutics for oncology. Licensing and collaborative research opportunities exist with respect to these biological materials.

    PMS2 is a weak mutator, the PMS control A12 has been engineered by adding an A onto the CRE. If a reversion to A11 occurs the Beta gal or Lacz reporter indicates an event of reversion. Such an event may turn over time into a tumor that warrants further characterization.

  • R26R-lox-stop-lox LacZ ( Mus musculus )

  • R26R-pCAGG lox-mTomato-lox mGFP ( Mus musculus )

  • SJR1973 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2R-10C lys2delta::hyg trp1delta ura3-52.

    Wild-type strain.

  • SJR1977 ( Saccharomyces cerevisiae )

    MATa ade2-101oc his4delta::LYS2F-10C lys2delta::hyg trp1delta ura3-52.

    Wild-type strain.

  • W1558 ( Saccharomyces cerevisiae )

    MATalpha ade2-1 CAN1 his3-11,15 leu2-3,112 trp1-1 ura3-1 RAD5

Reagents

  • Mouse Mlh-1 (-/-) Embryonic Cell Line ( Immortalized embryonic cell line )

    Phenotype: Mice deficient in Mlh-1 are mismatch repair deficient.

    OHSU patent #: OHSU # 0416.

  • Mouse PMS-2 (-/-) Embryonic Cell Line ( Immortalized embryonic cell line )

    Phenotype: Mice deficient in Pms2, a mammalian homolog of bacterial mutL, develop cancer and display microsatellite instability (MSI) in all tissues examined, including the male germ line where a frequency of approximately 10% was observed.

    OHSU patent #: OHSU # 0418.

    Males and females available.

Research Opportunities


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Last updated: 2012-07-02T12:41:29.502-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016