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Rosalie Sears Laboratory

Summary:

We are studying cellular signaling pathways involved in the generation of human cancer. In general, disruption of these pathways alters the ability of a cell to control its proliferation as well as the initiation of programmed cell death (apoptosis). We are focusing on three key signaling pathways that regulate both cellular proliferation and apoptosis: the Myc transcription factor, the Ras signaling protein, and the G1 cyclin dependent kinase (Cdk)/retinoblastoma(Rb)/E2F pathway. While each of these pathways has been extensively studied over the past decade, the nature of their interrelations remains elusive. Since these pathways are deregulated in the majority of all human tumors, we want to understand how they network and synergize to precisely control cellular proliferation versus cell death. This information will contribute to our understanding of the complex nature of cancer progression, and facilitate the generation of meaningful therapies.

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Organisms and Viruses

  • B56alpha+/- ( Mus musculus )

  • B56alpha-/-/RFS-MycWT/Lck-Cre   ( Mus musculus )

    Lck-cre transgenic mice contain a Cre-recombinase gene driven by the distal promoter of the lymphocyte protein tyrosine kinase (Lck) gene.

  • B56alpha-/-   ( Mus musculus )

  • BLG-Cre ( Mus musculus )

    BLG-Cre transgenic mice have the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation.

  • C57-KRAS(G12D)/p53(R172H)/Pdx1-Cre ( Mus musculus )

  • C57BL/6 ( Mus musculus )

  • EμSR-tTa ( Mus musculus )

    An immunoglobin enhancer inducing expression of the rtTA which then proceeds to subsequent doxycycline "inducibility".

  • EμSR-tTa/tet-o-myc ( Mus musculus )

  • FloxneoNeuNT ( Mus musculus )

  • FVB/NJ ( Mus musculus )

  • K5-CrePR1 ( Mus musculus )

    Cre recombinase progesterone receptor (PR) fusion protein under control of Keratin 5 promoter.

  • Lck-Cre ( Mus musculus )

    Lck-cre transgenic mice contain a Cre-recombinase gene driven by the distal promoter of the lymphocyte protein tyrosine kinase (Lck) gene.

  • LSL-KrasG12D ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

  • LSL-KrasG12D/P48-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    P48-Cre is a pancreas-specific Cre recombinase transgene.

  • LSL-KrasG12D/PDX1-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • Myc[S62A] ( Mus musculus )

  • NOD SCID gamma (c)(null) ( Mus musculus )

    Double homozygous for the severe combined immunodeficiency (SCID) mutation and interleukin-2Rgamma (IL-2Rgamma) allelic mutation (gamma(c)(null)).

  • P48-Cre ( Mus musculus )

    Pancreas-specific Cre recombinase transgene.

  • PDX1-Cre ( Mus musculus )

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • Pin+/- ( Mus musculus )

  • RFS- MycS62A/Lck-Cre ( Mus musculus )

    Lck-cre transgenic mice contain a Cre-recombinase gene driven by the distal promoter of the lymphocyte protein tyrosine kinase (Lck) gene.

  • RFS-myc[S62A]/Wap-cre ( Mus musculus )

    Wap-cre mice express Cre in the secretory luminal and ductal epithelial cells of the mammary gland during late pregnancy and lactation.

  • RFS-myc[T58A] ( Mus musculus )

  • RFS-myc[T58A]/K5-CrePR1/ TGFbetaRII[fx/fx] ( Mus musculus )

    Cre recombinase progesterone receptor (PR) fusion protein under control of Keratin 5 promoter.

  • RFS-myc[T58A]/Wap-cre ( Mus musculus )

    Wap-cre mice express Cre in the secretory luminal and ductal epithelial cells of the mammary gland during late pregnancy and lactation.

  • RFS-MycS62A/K5-CrePR1 ( Mus musculus )

    Cre recombinase progesterone receptor (PR) fusion protein under control of Keratin 5 promoter.

  • RFS-MycT58A+/+/LSL-KrasG12D ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

  • RFS-MycT58A+/+/LSL-KrasG12D/P48-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    P48-Cre is a pancreas-specific Cre recombinase transgene.

  • RFS-MycT58A+/+/P48-Cre ( Mus musculus )

    Pancreas-specific Cre recombinase transgene.

  • RFS-MycT58A+/+/PDX1-Cre ( Mus musculus )

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • RFS-MycT58A+/-/LSL-KrasG12D ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

  • RFS-MycT58A+/-/LSL-KrasG12D/Pdx1-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • RFS-MycT58A+/-/P48-Cre ( Mus musculus )

    P48-Cre is a pancreas-specific Cre recombinase transgene.

  • RFS-MycT58A+/-/PDX1-Cre ( Mus musculus )

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • RFS-mycT58A/BLG-Cre ( Mus musculus )

    BLG-Cre transgenic mice have the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation.

  • RFS-MycT58A/K5-CrePR1 ( Mus musculus )

    Cre recombinase progesterone receptor (PR) fusion protein under control of Keratin 5 promoter.

  • RFS-MycT58A/Lck-Cre ( Mus musculus )

    Lck-cre transgenic mice contain a Cre-recombinase gene driven by the distal promoter of the lymphocyte protein tyrosine kinase (Lck) gene.

  • RFS-MycT58A/LSL-KrasG12D/PDX1-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • RFS-mycT58A/NeuNT/BLG-Cre ( Mus musculus )

    BLG-Cre transgenic mice have the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation.

  • RFS-MycWT ( Mus musculus )

  • RFS-MycWT+/+/LSL-KrasG12D ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

  • RFS-MycWT+/+/LSL-KrasG12D/P48-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    P48-Cre is a pancreas-specific Cre recombinase transgene.

  • RFS-MycWT+/+/P48-Cre ( Mus musculus )

    P48-Cre is a pancreas-specific Cre recombinase transgene.

  • RFS-MycWT+/+/PDX1-Cre ( Mus musculus )

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas.

  • RFS-MycWT+/-/LSL-KrasG12D ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

  • RFS-MycWT+/-/LSL-KrasG12D/Pdx1-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • RFS-MycWT+/-/P48-Cre ( Mus musculus )

    P48-Cre is a pancreas-specific Cre recombinase transgene.

  • RFS-MycWT+/-/PDX1-Cre ( Mus musculus )

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas.

  • RFS-MycWT/BLG-Cre ( Mus musculus )

    BLG-Cre transgenic mice have the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation.

  • RFS-mycWT/K5-crePR1 ( Mus musculus )

    Cre recombinase progesterone receptor (PR) fusion protein under control of Keratin 5 promoter.

  • RFS-MycWT/K5-CrePR1/TGFbetaRIIfx/fx ( Mus musculus )

    Cre recombinase progesterone receptor (PR) fusion protein under control of Keratin 5 promoter.

  • RFS-MycWT/Lck-Cre ( Mus musculus )

    Lck-cre transgenic mice contain a Cre-recombinase gene driven by the distal promoter of the lymphocyte protein tyrosine kinase (Lck) gene.

  • RFS-MycWT/LSL-KrasG12D/PDX1-Cre ( Mus musculus )

    LSL-KrasG12D carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.

    Pdx-1-Cre mice exhibit a stochastic pattern of high-level Cre expression in the pancreas .

  • RFS-MycWT/NeuNT/BLG-Cre ( Mus musculus )

    BLG-Cre transgenic mice have the beta-lactoglobulin (BLG) promoter directing Cre recombinase expression to mammary gland during lactation.

  • RFS-mycWT/Wap-cre ( Mus musculus )

    Wap-cre mice express Cre in the secretory luminal and ductal epithelial cells of the mammary gland during late pregnancy and lactation.

  • RFS-mycWT/Wap-Cre/TGFbetaRIIfx/fx ( Mus musculus )

    Wap-cre mice express Cre in the secretory luminal and ductal epithelial cells of the mammary gland during late pregnancy and lactation.

  • RFS-Skp2 ( Mus musculus )

  • TGFbetaRIIfx/fx ( Mus musculus )

  • TRE-Axin-GFP ( Mus musculus )

  • Wap-Cre ( Mus musculus )

    Wap-cre mice express Cre in the secretory luminal and ductal epithelial cells of the mammary gland during late pregnancy and lactation.

  • Wap-Cre/TGFbetaRIIfx/fx ( Mus musculus )

    Wap-cre mice express Cre in the secretory luminal and ductal epithelial cells of the mammary gland during late pregnancy and lactation.

  •  B56alpha+/+ ( Mus musculus )

    PP2A B56alpha subunit wild type strain.

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Last updated: 2012-12-10T14:28:38.947-06:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016