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Claudio Mello Laboratory

Summary:

Research interests include:
Neuroethology, molecular neuroscience, vocal communication, vocal learning, central auditory processing, learning and memory, neurogenomics, neuronal and synaptic plasticity, activity-dependent gene regulation, avian physiology, comparative neuiroscience, sex steroid actions on the brain and behavior, adult neurogenesis.

Affiliations:

People:

    Resources:

    Instruments

    • High througput in situ hybridization workstation ( In situ hybridization workstation )

    • Leica CM1850 cryostat ( Cryostat )

      "The Leica CM1850 cryostat design incorporates emphasis on power savings, increased efficiency and operator safety.

      The result is a versatile cryostat with an optimized cooling system, rapid specimen freezing and smooth specimen orientation for the high-quality sectioning demanded in routine histology and clinical pathology."

    • Nikon E600 microscope ( Microscope )

      Equipped with brightfield, darkfield, or fluorescenct optics, a motorized stage drive and a digital camera (DVC, Austin, TX) and contains a X-Y-Z stage encoder (Ludl Electronic Products, Hawthorne, NY) and a Lucivid-illuminated camera lucida extension to a microcomputer equipped with Neurolucida software (MicroBrightField, Colchester, VT).

    • Sterotaxic device for small animals ( Stereotaxic device )

    Organisms and Viruses

    • Transgenic Zebra Finch ( Taeniopygia guttata )

      Transgenic zebra finch lines are being developed by laboratory. Contact lab for details.

    • Zebra finch ( Taeniopygia guttata )

    Protocols

    Reagents

    • anti-DIG antibody ( Polyclonal antibody reagent )

      Use at 1:300 dilution.

    • Gad65 riboprobes ( RNA probe )

      Radioactive and non-radioactive probes. Fluorescein-, Digoxygenin (DIG)- or 33P-labeled riboprobes.

    • Zebra Finch gene library ( Construct library )

      "The Songbird EST project is a database for finding and retrieving gene sequences expressed in the zebra finch brain."

    • Zebra finch zRalDH digoxigenin-labeled riboprobes ( RNA probe )

      Primer sequences: 3'-TAATACGACTCACTATAGGG ACAGCGTAGTC-5' for T7;
      5' -ATTAACCCTCACTAAAGGGAGAATCACTGAACAGCG-3'

    Services

    Software

    • Adobe Illustrator ( Software )

      "Adobe® Illustrator® CS5 software provides precision and power with sophisticated drawing tools, expressive natural brushes, a host of time-savers, and integration with Adobe CS Live online services."

    • Adobe Photoshop ( Software )

      "Photoshop is the professional standard for editing and manipulating digital images, ideal for photographers, graphic designers, and web designers."

    • ImageJ ( Software )

      "ImageJ is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.4 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux.

      It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and "raw". It supports "stacks", a series of images that share a single window. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.

      It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. It can create density histograms and line profile plots. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering."

    • JMP ( Software )

      "Data visualization and deep analytics for the desktop – software that now reaches out to users of Microsoft Excel, R and advanced data mining and modeling techniques."

    • Neurolucida ( Software )

      "Neurolucida is advanced scientific software for brain mapping, neuron reconstruction, anatomical mapping, and morphometry."

    • SlideBook ( Software )

      "Comprehensive Digital Microscopy Package: SlideBook software carries biomedical imaging through the entire experimental process. By addressing everything from device control to image processing to automated data analysis, SlideBook allows users to focus on scientific investigation and novel methods rather than on core instrument functionality."


    Web Links:

    Last updated: 2011-03-31T15:26:34.075-05:00

    Copyright © 2016 by the President and Fellows of Harvard College
    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016