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Owen McCarty Laboratory

Summary:

Dr. McCarty's research is focused on understanding the interplay between cell biology and fluid mechanics in the cardiovascular system. In particular, his research into the balance between hydrodynamic shear forces and chemical adhesive interactions has great relevance to underlying processes of cancer, cardiovascular disease, and inflammation.

Affiliations:

People:

Resources:

Biological Specimens

Instruments

  • Molecular Devices SpectroMax 340 absorbance microplate reader ( Spectrophotometer )

    "The patented multichannel optical design of the SpectraMax® 340PC384 Absorbance Microplate Reader mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans."

  • Trinity Biotech KC4 coagulation analyzer ( Coagulation analyzer )

    The KC4D Coagulation analyzer has the following features:
    "*Open system with true clot detection using the proprietary Ball Method
    *Programmable single and duplicate test modes (0-300 sec.)
    *Minimum sample volume, 0.15m
    *Incubation area at 37°C: eight samples, five reagents, two pipets
    *RS-232 interface"

  • Zeiss Axiovert 200 M with a Zeiss plan APO 63X oil immersion objective ( Inverted microscope )

    The Zeiss plan APO (apochromat lens) 63X oil immersion objective is for 160mm tube length microscopes and and has numerical aperture (NA) 1.40.

Protocols

  • Immunohistology for platelet adhesion ( Protocol )

    Referenced in:
    O.J.T. McCarty, R.B. Conley, W. Shentu et al., (2010) Molecular imaging of activated von Willebrand factor to detect high-risk atherosclerotic phenotype, J Am Coll Cardiol Img 3:947–955.

Reagents

Software

  • ImageJ ( Software )

    "ImageJ is a public domain Java image processing program inspired by NIH Image for the Macintosh. It runs, either as an online applet or as a downloadable application, on any computer with a Java 1.4 or later virtual machine. Downloadable distributions are available for Windows, Mac OS, Mac OS X and Linux.

    It can display, edit, analyze, process, save and print 8-bit, 16-bit and 32-bit images. It can read many image formats including TIFF, GIF, JPEG, BMP, DICOM, FITS and "raw". It supports "stacks", a series of images that share a single window. It is multithreaded, so time-consuming operations such as image file reading can be performed in parallel with other operations.

    It can calculate area and pixel value statistics of user-defined selections. It can measure distances and angles. It can create density histograms and line profile plots. It supports standard image processing functions such as contrast manipulation, sharpening, smoothing, edge detection and median filtering."


Last updated: 2011-05-05T14:25:36.092-05:00

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The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016