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Andras Gruber Laboratory

Summary:

Current Research Goals:
To determine the role of contact activation in acute intraluminal thrombus propagation using synthetic vascular grafts in a primate model of thrombosis and hemostasis. If the functionality of the contact system enzyme complex (FXI/FXII/KK/HMWK) is relevant to the pathogenesis of thrombosis, a FXI inhibitor could become the first safe antithrombotic agent.

To characterize the effects of endogenous protein C activation on acute arterial thrombogenesis and hemostasis using rationally engineered recombinant enzymes. A pharmacologically viable protein C activator could help utilize the body's own antithrombotic and antiinflammatory system similar to the way streptokinase and tPA became useful fibrinolysis activators.

Affiliations:

People:

Resources:

Biological Specimens

Instruments

  • Electromechanical fibrometer ( Fibrometer )

  • GE-400T gamma scintillation camera ( Gamma camera )

    Interfaced with a NuQuest InteCam computer system.

  • Hemavet HV950FS analyzer ( Hematology analyzer )

    "The HEMAVET® provides superior cell separation for accurate and precise analysis through use of patented Focused Flow Technology (eleven patents issued) thus giving the best technology available for dealing with veterinary samples. In addition patented Expectation Maximization software helps categorize the different types of cells."

  • Molecular Devices SpectroMax 340 microplate reader ( Microplate reader )

    The SpectroMax 340 "mimics a dual beam spectrophotometer, measures each sample in the plate directly, and eliminates measurement error due to variations in light output between optical fibers on other instruments for more reliable data reporting. The eight-channel system delivers both high precision and speed when reading 96- and 384-well microplates for endpoint measurements, kinetic assays, and spectral scans."

  • Serono System 9000 Hematology Series cell counter ( Automatic cytometer )

Organisms and Viruses

Protocols

  • Murine arterial thrombosis model ( Protocol )

    Model used to study thrombosis formation in mouse models. Thrombus formation induced by ferric chloride or laser injury.

  • Murine peritoneal sepsis model ( Protocol )

    Model is used to study the role of anticoagulation in the treatment of sepsis-associated consumptive coagulopathy.

Reagents


Web Links:

Last updated: 2012-07-02T14:03:10.578-05:00

Copyright © 2016 by the President and Fellows of Harvard College
The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016