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Charles Roselli Laboratory

Summary:

My research goal is to obtain an integrated understanding of the steroid-sensitive brain circuitry, neurochemical mechanisms, and the subcellular signaling pathways that mediate the central actions of androgens. A major research focus is the characterization of the aromatase-signaling pathway in neural tissue. Aromatase is a cytochrome P450 enzyme (CYP 19) that catalyzes the conversion of testosterone to estradiol. Although androgens and brain-derived estrogens are believed to act coordinately to regulate brain function, the challenge is to understand the complexity of this interaction at a subcellular, cellular, and systems level. A second research direction in my laboratory aims to understand the cellular mechanism(s) by which herbal preparations inhibit prostate growth and to explore their potential as viable treatments for prostate carcinoma.

People:

      Member: Roselli, Charles, PhD
      Role: Professor of Anesthesiology, Professor of Physiology and Pharmacology

    Resources:

    Biological Specimens

    Instruments

    Organisms and Viruses

    • Ovis aries ( Ovis aries )

      Mixed Western breeds were purchased from local farmers or bred at the sheep facility at Oregon State University, Corvalis, Oregon.

    Protocols

    Reagents

    • Curcumin ( Organic small molecule )

      "A natural phenolic compound. Potent anti-tumor agent having anti-inflammatory and anti-oxidant properties. Induces apoptosis in cancer cells and inhibits phorbol ester-induced protein kinase C (PKC) activity. Reported to inhibit production of inflammatory cytokines by peripheral blood monocytes and alveolar macrophages. Potent inhibitor of EGFR tyrosine kinase and IκB kinase. Inhibits inducible nitric oxide synthase (iNOS), cycloxygenase and lipoxygenase. Easily penetrates into the cytoplasm of cells, accumulating in membranous structures such as plasma membrane, endoplasmic reticulum and nuclear envelope."

    • Gö 6976 ( Organic small molecule )

      "Gö 6976 is an inhibitor of protein kinase C (PKC) specific for α (IC50=2.3 nM) and β1 (IC50=6.2 nM) isozymes."

    • PC-3 prostate cancer cell line ( Tumor-derived cell line )

    • Phorbol 12-myristate 13-acetate ( Organic small molecule )

      "Potent tumor promoter; activates protein kinase C in vivo and in vitro."

    • Recombinant CCL2 ( Purified protein reagent )

    • RS 102895 hydrochloride ( Organic small molecule )

      "RS 102895 hydrochloride is a chemokine receptor CCR2 antagonist."

    • Testosterone Propionate ( Organic macromolecule )

    Software

    • Image J ( Software )

      "ImageJ is a public domain, Java-based image processing program developed at the National Institutes of Health. ImageJ was designed with an open architecture that provides extensibility via Java plugins and recordable macros. Custom acquisition, analysis and processing plugins can be developed using ImageJ's built-in editor and a Java compiler. User-written plugins make it possible to solve many image processing and analysis problems, from three-dimensional live-cell imaging, to radiological image processing, multiple imaging system data comparisons to automated hematology systems. ImageJ's plugin architecture and built in development environment has made it a popular platform for teaching image processing.

      ImageJ can be run as an online applet, a downloadable application, or on any computer with a Java 5 or later virtual machine. Downloadable distributions are available for Microsoft Windows, Mac OS, Mac OS X, Linux, and the Sharp Zaurus PDA. The source code for ImageJ is freely available."

    • Prism ( Software )

      "GraphPad Prism is a powerful combination of biostatistics, curve fitting (nonlinear regression) and scientific graphing in one comprehensive program. Easily organize, analyze and graph repeated experiments; pick appropriate statistical tests and interpret the results."


    Web Links:

    Last updated: 2011-06-07T13:39:39.107-05:00

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    The eagle-i Consortium is supported by NIH Grant #5U24RR029825-02 / Copyright 2016