Histopathology Shared Resource

Director: Thomas, George, M.D.

Summary:

The Histopathology Shared Resource is a College of American Pathologists (CAP) certified and Clinical Laboratory Improvement Amendments (CLIA) accredited core facility that provides investigators with tissue-based services fundamental to the progress of basic, translational and clinical research.

The mission the Histopathology Shared Resource (HSR) is to:

1. Provide investigators with histology services critical for the progress of biomedical research involving studies on research animal and human tissues These services include cryostat sectioning, paraffin- embedding, cell pellet and cytospin preparation, microtome-sectioning, histochemical staining and immunohistochemistry.

2. Provide investigators with consultation and education in regard to best use of histopathology services research protocols, safety issues, and IRB application.

Affiliations:

• Knight Cancer Institute

People:

• Director: Thomas, George, M.D.
  

Member: Zarceno, Joscelyn
Role: Laboratory assistant

Resources:

Instruments

• ABI GeneAmp 9700 96-well PCR system ( PCR instrument )
  

  "GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications." There are three instruments in this lab. Instrument has dual 384 well plates.

• Eppendorf AG epMotion 5075 liquid handler ( Liquid handler )
  

• Leica Autostainer XL slide stainer ( Automatic staining machine )
  

  "The Leica Autostainer XL is the ideal solution for automated, routine slide staining in histology and cytology."

• Leica DM LB light microscope ( Optical microscope )
  

• Leica RM2165 microtome ( Microtome )
  

• Microm HM 505 E cryostat ( Cryostat )
  

• Sakura Tissue-Tek SCA automatic coverslipper ( Automatic coverslipper )
  

• Sakura Tissue-Tek TEC paraffin embedding station ( Tissue embedding station )
  

• Sakura Tissue-Tek VIP tissue processor ( Automatic tissue processor )
  

• Thermo Scientific Shandon Cryotome FE cryostat ( Cryostat )
  

• Thermo Scientific Shandon Finesse ME microtome ( Microtome )
  

• Ventana Discovery Autostainer ( Automatic staining machine )
  

Services

• Histology tissue and staining services ( Material processing service )
  

  "This core facility is equipped and staffed to provide a full array of histology services including Tissue processing and paraffin embedding, Sectioning (cryostat or paraffin-embedded) to specified thickness and Histochemical stains, such as:
  
  Hematoxylin and eosin (H&E)
  Trichrome
  Elastin, Reticulin
  Giemsa
  Luxol fast blue
  Iron
  Bacterial and fungal"

• Immunohistochemistry staining service ( Material processing service )
  

  "The Histopathology Shared Resource provides Immuhistochemistry services which include:
  
  Testing and titering new antibodies, both monoclonal and polyclonal. The latter should be either a purified IgG fraction or affinity-purified. (Straight serum is not suitable for immunostaining).
  Optimization of staining to enhance detection and reduce background.
  Epitope retrieval techniques (pre-treatment with heat and/or proteases)
  Specialized secondary detection reagents
  Avidin/biotin blocking steps
  Double- and triple-antibody stains using multiple chromagens."

Web Links:

• http://www.ohsu.edu/xd/health/services/cancer/resea
  rch-training/shared-resources/cancer-pathology.cfm
• http://www.ohsu.edu/xd/research/research-cores/histopathology/