The Histopathology Shared Resource is a College of American Pathologists (CAP) certified and Clinical Laboratory Improvement Amendments (CLIA) accredited core facility that provides investigators with tissue-based services fundamental to the progress of basic, translational and clinical research.
The mission the Histopathology Shared Resource (HSR) is to:
1. Provide investigators with histology services critical for the progress of biomedical research involving studies on research animal and human tissues These services include cryostat sectioning, paraffin- embedding, cell pellet and cytospin preparation, microtome-sectioning, histochemical staining and immunohistochemistry.
2. Provide investigators with consultation and education in regard to best use of histopathology services research protocols, safety issues, and IRB application.
Role: Laboratory assistant
"GeneAmp® PCR System 9700 is specifically designed for the amplification of nucleic acids. The GeneAmp® PCR System 9700 consists of a base module and one of many interchangeable sample block modules. The 96-well GeneAmp® PCR System 9700 is designed for use with 0.2 ml reaction tubes or 96-well reaction plates for all of your routine PCR applications." There are three instruments in this lab. Instrument has dual 384 well plates.
"The Leica Autostainer XL is the ideal solution for automated, routine slide staining in histology and cytology."
"This core facility is equipped and staffed to provide a full array of histology services including Tissue processing and paraffin embedding, Sectioning (cryostat or paraffin-embedded) to specified thickness and Histochemical stains, such as:
Hematoxylin and eosin (H&E)
Luxol fast blue
Bacterial and fungal"
"The Histopathology Shared Resource provides Immuhistochemistry services which include:
Testing and titering new antibodies, both monoclonal and polyclonal. The latter should be either a purified IgG fraction or affinity-purified. (Straight serum is not suitable for immunostaining).
Optimization of staining to enhance detection and reduce background.
Epitope retrieval techniques (pre-treatment with heat and/or proteases)
Specialized secondary detection reagents
Avidin/biotin blocking steps
Double- and triple-antibody stains using multiple chromagens."