Portland Alcohol Research Center

Summary:

The center supports the coordinated research efforts of 20 scientists at the VA and OHSU, and one scientist at the University of Pittsburgh, in areas ranging from behavioral neuroscience to molecular biology. A major emphasis of the research is using animal models to study the genetic contributions to alcohol and drug sensitivity. The PARC is focused on the genetics of how the brain adapts to alcohol. Genetic mapping techniques have allowed a large group of investigators collaboratively to identify the location of individual genes affecting responses to alcohol in mice. The PARC is unique in its focus on gene mapping and discovering the function of many such genes relevant for how the brain responds to chronic alcohol.

Affiliations:

• Department of Behavioral Neuroscience
• Portland VA Medical Center

People:

Member: Metten, Pamela, Ph.D.
Role: Co-Director, Animal & Phenotyping Core, Portland Alcohol Research Center, Research Geneticist, Portland VA Medical Center, Senior Research Associate

Resources:

Organisms and Viruses

• B6.D2 Chr 1 MtvTy/7a (D1MIT113,291) ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (173.6 - 186.4 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2 ISCS12 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (172.8 - 174.4 Mb) congenic on a C57BL/6J (B6) background.
  
  Congenic strain, interval specific.

• B6.D2 ISCS6 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (172.9 - 176.3 Mb) congenic on a C57BL/6J (B6) background.
  
  Congenic strain, interval specific.

• B6.D2 ISCS8 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (172.9 - 174.0 Mb) congenic on a B6 background.
  
  Congenic strain, interval specific.

• B6.D2 ISCS9 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (174.4 - 176.3 Mb) congenic on a C57BL/6J (B6) background.
  
  Congenic strain, interval specific.

• B6.D2-D1MIT200,150 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (152.0 - 176.4 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D2MIT80,285 congenic ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 2 segment (20.9 -152.5 Mb) congenic on a C57BL/6J (B6) background. Available live and cryopreserved.
  
  Congenic strain.

• B6.D2-D2MIT80,285 control ( Mus musculus )
  

  Used to study alcoholism. Control strain for DBA/2J (D2) chromosome 2 segment (20.9 -152.5 Mb) congenic on a C57BL/6J (B6) background. Available live and cryopreserved.
  
  Congenic control strain.

• B6.D2-D2MIT91,164 congenic ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 2 segment (66.5 - 123.1Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D2MIT91,164 control ( Mus musculus )
  

  Used to study alcoholism. Control strain for DBA/2J (D2) chromosome 2 segment (66.5 - 123.1Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D9MIT129,71 congenic ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 9 segment (43.6 - 50.0 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D9MIT129,71 control ( Mus musculus )
  

  Used to study alcoholism. Control strain for DBA/2J (D2) chromosome 9 segment (43.6 - 50.0 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D9MIT31,144 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 9 segment (63.6 - 63.8 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D9MIT90,18 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 9 segment (32.6 - 120.1 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-D9MIT90,182 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 9 segment (32.6 - 101.4 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-R4 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (172.9 - 177.9 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• B6.D2-R8 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 1 segment (172.9 - 174.0 Mb) congenic on a C57BL/6J (B6) background. Cryopreserved.

• COLD-1 mouse ( Mus musculus )
  

  Used to study alcoholism; alcohol-induced hypothermia test. Shows profound hypothermia. Embryo cryopreserved. Selectively bred line.

• COLD-2 mouse ( Mus musculus )
  

  Used to study alcoholism; alcohol-induced hypothermia test. Shows profound hypothermia. Embryo cryopreserved. Selectively bred line.

• D1.D2 D11Mit217-140 ( Mus musculus )
  

  Used to study alcoholism. DBA/2J (D2) chromosome 11 segment (37.4 - 54.0 Mb) congenic on a DBA/1J (D1) background.

• D2.B6 Chr 4 ISCS 4 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 4 segment (31.3 - 75.1 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6 D11Mit82-258 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 11 segment (2-65 cM) congenic on a DBA/2J (D2) background.

• D2.B6 ISCS2 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 1 segment (169.2 - 173.3 Mb) congenic on a DBA/2J (D2) background.
  
  Congenic strain, interval specific.

• D2.B6 ISCS3 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 1 segment (169.2 - 176.4 Mb) congenic on a DBA/2J (D2) background.

• D2.B6-Chr4 ISCS2 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 4 segment (53.6 - 81 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6-D11MIT78,179 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 11 segment (17.8 - 89.6 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6-D11MIT82,258 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 11 segment (25.9 - 107.5 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6-D19MIT109,137 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 19 segment (6.1 - 59 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6-D19MIT13,100 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 19 segment (32.3 - 42.3 Mb) congenic on a DBA/2J (D2) background.

• D2.B6-D19MIT13,91 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 19 segment (32.3 - 47.8 Mb) congenic on a DBA/2J (D2) background.

• D2.B6-D19MIT19,91 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 19 segment (39.3 - 47.8 Mb) congenic on a DBA/2J (D2) background.

• D2.B6-D19MIT70,55 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 19 segment (50.6 - 55.2 Mb) congenic on a DBA/2J (D2) background.

• D2.B6-D1MIT200,150 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 1 segment (152.0 - 176.4 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6-D2MIT91,164, congenic ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 2 segment (66.5 - 123.1 Mb) congenic on a DBA/2J (D2) background.

• D2.B6-D2MIT91,164, control ( Mus musculus )
  

  Used to study alcoholism. Control strain for C57BL/6J (B6) chromosome 2 segment (66.5 - 123.1 Mb) congenic on a DBA/2J (D2) background.
  
  Congenic control strain.

• D2.B6-D9MIT90,18 ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 9 segment (31.3 - 120.1 Mb) congenic on a DBA/2J (D2) background. Available live and cryopreserved.

• D2.B6-mpdz ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 4 segment (75.1 - 76.5 Mb) congenic on a DBA/2J (D2) background. Cryopreserved.

• D2.B6-Tyrp1(D4MIT192,327) ( Mus musculus )
  

  Used to study alcoholism. C57BL/6J (B6) chromosome 4 segment (31.1 - 86.1 Mb) congenic on a DBA/2J (D2) background. Available live and cryopreserved.

• D2.D1 D11Mit217-140 ( Mus musculus )
  

  Used to study alcoholism. DBA/1J (D1) chromosome 11 segment (37.4 - 54.0 Mb) congenic on a DBA/2J (D2) background.

• FAST-1 mouse ( Mus musculus )
  

  Used to study alcoholism; enhanced locomotor stimulant response to alcohol. Cryopreserved. Selectively bred line.

• FAST-2 mouse ( Mus musculus )
  

  Used to study alcoholism; enhanced locomotor stimulant response to alcohol. Cryopreserved. Selectively bred line.

• HAFT-1 mouse ( Mus musculus )
  

  Used to study alcoholism; acute functional tolerance to alcohol. Embryo cryopreserved. High Acute Functional Tolerance -1. Selectively bred line.

• HDID-1 mouse ( Mus musculus )
  

  Used to study alcoholism, alcohol binge drinking. Selectively bred line.

• HDID-2 mouse ( Mus musculus )
  

  Used to study alcoholism, and alcohol binge drinking. High Drinking in Dark (HDID)-2. Selectively bred line.

• HOT-1 mouse ( Mus musculus )
  

  Used to study alcoholism; alcohol-induced hypothermia test. Displays mild to no hypothermia. Embryo cryopreserved. Selectively bred line.

• HOT-2 mouse ( Mus musculus )
  

  Used to study alcoholism, alcohol-induced hypothermia test. Displays mild to no hypothermia. Embryo cryopreserved. Selectively bred line.

• IWSP-1 mouse ( Mus musculus )
  

  Used to study alcoholism; severe withdrawal convulsions after chronic dependence on alcohol. Sperm are cryopreserved. Fully inbred. Inbred strain from Withdrawal Seizure Prone - 1, derived from selectively bred line.

• IWSP-2 mouse ( Mus musculus )
  

  Used to study alcoholism; severe withdrawal convulsions after chronic dependence on alcohol. Fully inbred. Inbred strain from Withdrawal Seizure Prone-2, derived from selectively bred line. Live mice are available.

• IWSR-1 mouse ( Mus musculus )
  

  Used to study alcoholism; reduced withdrawal convulsions after chronic dependence on alcohol. Inbred strain from Withdrawal Seizure Resistant - 1. Fully inbred, derived from selectively bred line. Live mice are available.

• IWSR-2 mouse ( Mus musculus )
  

  Used to study alcoholism, reduced withdrawal convulsions after chronic dependence on alcohol. Sperm are cryopreserved. Inbred strains from Withdrawal Seizure Resistant - 2. Fully inbred, derived from selectively bred line.

• LAFT-1 mouse ( Mus musculus )
  

  Used to study alcoholism; acute functional tolerance to alcohol. Low Acute Functional Tolerance - 1. Embryo cryopreserved.
  
  Selectively bred line.

• SLOW-1 mouse ( Mus musculus )
  

  Used to study alcoholism; displays a reduced stimulant response to alcohol relative to controls. Cryopreserved. Selectively bred line.

• SLOW-2 mouse ( Mus musculus )
  

  Used to study alcoholism; displays a reduced stimulant response to alcohol relative to controls. Cryopreserved.

• WSC-1 mouse ( Mus musculus )
  

  Used to study alcoholism. Randomly bred controls in parallel with WSP and WSR. Withdrawal Seizure Control - 1.
  
  Heterogeneous stock.

• WSC-2 mouse ( Mus musculus )
  

  Used to study alcoholism. Randomly bred controls in parallel with WSP and WSR. Withdrawal Seizure Control - 2.
  
  Heterogeneous stock.

• WSP-1 mouse ( Mus musculus )
  

  Used to study alcoholism; severe withdrawal convulsions after chronic dependence on alcohol. Withdrawal Seizure Prone - 1.
  
  Selectively bred line.

• WSP-2 mouse ( Mus musculus )
  

  Used to study alcoholism; severe withdrawal convulsions after chronic dependence on alcohol. Withdrawal Seizure Prone - 2.
  
  Selectively bred line.

• WSR-1 mouse ( Mus musculus )
  

  Used to study alcoholism; reduced withdrawal convulsions after chronic dependence on alcohol. Withdrawal Seizure Resistant - 1.
  
  Selectively bred line.

• WSR-2 mouse ( Mus musculus )
  

  Used to study alcoholism; decreased withdrawal convulsions after chronic dependence on alcohol. Withdrawal Seizure Resistant - 2.
  
  Selectively bred line.

Protocols

• Drinking in the Dark (DID): SOP for the Mouse ( Protocol )
  

  This document summarizes two variations of the "standard" Drinking in the Dark method as we use it for C57BL/6J mice.

Services

• Binge drinking test ( Training service )
  

• Drug withdrawal test training service ( Training service )
  

Web Links:

• http://www.ohsu.edu/parc/