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|  Oregon Health and Science University |
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| Oregon Health and Science University |
The Endocrine Technology Support Core Lab (ETSL) has provided intra- and extramural investigators with analysis of protein and steroid content in blood, as well as in biological tissues and fluids for more than 3 decades.
Principal support functions include provision of routine assays (for most naturally occurring steroid and protein hormones involved in the regulation of reproduction, metabolism, and the central nervous system) and development of new assays (e.g., Multiplex platforms for cytokines and signaling molecules).
Member:
Blue, Steven
Role:
Senior Research Assistant
Member:
Winchell, Andrea
Role:
Research Assistant 2
This lab shares the instrument's usage with another lab which owns it.
"ECL-based immunoassay analyser for accurate first-time results
The cobas e 411 is a fully automated, multi-channel analyser employing sophisticated electrochemiluminescence (ECL) technology to deliver excellent low-end sensitivity and broad dynamic range. With an intuitive touch-screen user-interface, it offers a convenient customisable solution for rapid quality-controlled results."
This lab shares the instrument's usage with another lab which owns it.
"The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates."
To isolate and purify various steroids in ether extracts of tissue samples, serum, plasma or other biological materials from a variety of species before radioimmunoassay (RIA).
To extract small volumes of serum, plasma, or cell culture media in ethanol prior to steroid assay.
Steroid assay:To determine the concentration of steroid hormones in ether or ethanol extracted serum, plasma, and cell culture media samples from macaques, humans, or other species using an overnight incubation.
To ether extract serum or plasma prior to steroid assay.
Steroid assay:To determine the concentration of steroid hormones in ether or ethanol extracted serum, plasma, and cell culture media samples from macaques, humans, or other species using an overnight incubation.
Procedure for properly euthanizing mice; testes removal; extraction of Leydig Cells
To determine whether the bovine conceptus synthesized and secreted a substance having luteinizing hormone (LH) activity.
To extract small volumes of serum, plasma, or cell culture media in methanol
Vasopressin: peptide hormone
Peptide quantitation method - amino acid analysis. If there is a tyrosine, peptide iodination assay is good, Bradford (protein assay) will not work.
General preparation instructions for reagents used in regular, in-house steroid Radioimmunoassay (RIA).
Steroid assay: To determine the concentration of steroid hormones in ether or ethanol extracted serum, plasma, and cell culture media samples from macaques, humans, or other species using an overnight incubation.
To determine the concentration of steroid hormones in ether or ethanol extracted serum, plasma, and cell culture media samples from macaques, humans, or other species using an overnight incubation.
To determine the concentration of estradiol in serum or plasma samples from rats, mice, rabbits or other species with normal estradiol concentrations of < 50 pg/ml using non-equibrium RIA conditions to enhance sensitivity of the standard curve.
"Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Cytokine assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Cytokine assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits."
Metabolic hormone assays for endocrine system
Metabolic hormone assay for endocrine system "Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide."
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide."
Metabolic hormone assay for endocrine system "Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide."
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Liquid Chromatography for multiple steroids: Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Metabolic hormone assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Metabolic hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Neurotransmiter assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Neurotransmiter assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Neurotransmiter assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Neurotransmiter assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Neurotransmitter assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Neurotransmitter assay for endocrine system
"Specific assay: Specific assay for proteins or steroids in body fluids or components, i.e., saliva, urine, ovary, semen, hair, or embryos of various species, including fish, reptiles and mammals." Petide hormone assays for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assays for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assays for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assays for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Peptide hormone assays for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Peptide hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Peptide hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Liquid Chromatography for multiple steroids: Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Protein hormone assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Liquid Chromatography for multiple steroids: Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Protein hormone assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Protein hormone assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Protein hormone assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Steroid assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"LH Bioassay: A mouse Leydig cell bioassay is available to measure serum LH levels in the monkey, particularly for small sample volumes collected for analyzing pulsatile LH patterns or in samples with LH concentrations too low to be detected by a specific cynomolgus monkey LH RIA." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Liquid Chromatography for multiple steroids: Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Steroid assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Liquid Chromatography for multiple steroids: Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Steroid assay for endocrine system
"Liquid Chromatography for multiple steroids: Glass columns with Sephadex LH-20 are used to separate steroid hormones, including estradiol-17ß, testosterone, dihydrotestosterone, androstenedione, and progesterone, in a single sample with limited volume. When necessary, chromatography is also used for purification of samples from different species before processing with steroid RIAs." Steroid assay for endocrine system
"Extraction assay for single steroid: Many steroid hormones in serum or plasma can be extracted either by ethanol or ether for RIA, including estradiol-17ß, estrone, estriol, testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone (DHEA) progesterone, 17OH-progesterone, corticosterone, ethinylestradiol (EE2), and Levonorgestrel (LNG)." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Immunochemiluminescence assay: The Roche Cobas e411 automatic clinical platform currently has 40 assays available for human serum. The following assays have also been validated for monkey serum: estradiol-17ß, progesterone, testosterone, cortisol, ACTH, prolactin, TSH, total T3, free T3, total T4, free T4, insulin, and C-Peptide." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
"Direct assay: Steroid, protein, or peptide assay without extraction are available as in-house assays or from commercial sources, including monkey LH, FSH, inhibin-B, growth hormone, prolactin, leptin, ghrelin, glucagons, IGF-1, total or high molecular weight adiponectin, pregnenolone, cotinine, and other commercial assay kits." Steroid assay for endocrine system
