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|  Oregon Health and Science University |
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| Oregon Health and Science University |
Access to EM Core equipment and services is available to all researchers at OHSU. We also provide EM services to non-OHSU institutions and individuals. Our intention is to make services available at reasonable rates. To maximize our time and to minimize costs, researchers may participate in sample preparation, sectioning, and/or EM viewing steps. Training for equipment usage is provided at an hourly rate, and individuals can schedule access to equipment after they are trained. We invite anyone interested in using EM approaches for pilot projects, or ongoing investigations to consult with us on their needs, protocols, services, and cost estimates.
Member:
Lopez, Claudia
Member:
Webb, Mike
Comes with Gatan 626 cryostage and 794 CCD camera.
"The system is based on the world-class modular Titan™ TEM technology in mechanical, electronic and thermal stability and is designed to deliver the ultimate performance in both life science and materials science applications. The Titan Krios has been optimized for cryo-transmission electron microscopy, automated single particle analysis, 2D electron crystallography and dual-axis tomography. The revolutionary capabilities of the Titan Krios open the gates to the investigational frontier of biological sciences, presenting unprecedented opportunities for ground-breaking results and breakthrough discoveries."
"The Tecnai™ Spirit is an easy-to-use TEM designed to provide high-contrast, high-resolution imaging and analysis in life science applications. Its unique BioTWIN lens is designed for optimal contrast and allows the observation of unstained material. Sophisticated automation allows you to concentrate on scientific challenges rather than the details of operating the instrument. A series of embedded side-mount and bottom-mount CCD cameras provide instant, high-quality digital results at the push of a button."
"The system is based on the world-class modular Titan™ TEM technology in mechanical, electronic and thermal stability and is designed to deliver the ultimate performance in both life science and materials science applications. The Titan Krios has been optimized for cryo-transmission electron microscopy, automated single particle analysis, 2D electron crystallography and dual-axis tomography. The revolutionary capabilities of the Titan Krios open the gates to the investigational frontier of biological sciences, presenting unprecedented opportunities for ground-breaking results and breakthrough discoveries."
The automatic critical point dryer can be pre-programmed or use operator controlled cycling. Other features include integrated temperature control and specimen chamber.
- Analysis of protein assemblies, macromolecular complexes, nucleic acids
- Negative stain, dark field and cryo EM
- Tomography
- Image analysis, averaging and reconstructions
Service includes:
• Sample preparation
• Fixation, embedding, sectioning and staining
• EM imaging, digital and print micrographs
• Antibody and gold-conjugate incubations
• Correlative integrated light-EM (iLEM)
Service includes:
• Sample fixation/dehydration
• Critical-point drying
• Sputter coating
• Imaging
Service includes:
• Sample preparation
• Fixation, embedding, sectioning and staining
• EM imaging, digital and print micrographs