Immunogold localization and correlative light-electron microscopy

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   Service includes: • Sample preparation • Fixation, embedding, sectioning and staining • EM imaging, digital and print micrographs • Antibody and gold-conjugate incubations • Correlative integrated light-EM (iLEM)

3. Service Fee URL

   http://www.ohsu.edu/xd/education/schools/school-of-medicine/departments/basic-science-departments/molecular-microbiology-and-immunology/resources/upload/Immunogold.jpg

4. Contact

   Barklis, Eric, Ph.D.

5. Related Resource

   Philips/FEI CM120 Biotwin transmission electron microscope

6. Related Resource

   Philips/FEI Technai G2 Spirit integrated light-electron microscope (iLEM)

7. Related Resource

   Philips/FEI Titan Krios Cryo-electron microscope

8. Related Resource

   Philips/FEI Helios 650 Dual Beams scanning electron microscope

9. Access Restriction(s)

   "Access to EM Core equipment and services is available to all researchers at OHSU. We also provide EM services to non-OHSU institutions and individuals."

10. Service Provided by

    Electron Microscropy Core

11. Related Technique

    Electron microscopy

12. Related Technique

    Tissue embedding

13. Related Technique

    Tissue sectioning

14. Related Technique

    Tissue fixation

15. Related Technique

    Immunogold labeling

16. Related Technique

    Sample preparation for assay

17. Related Technique

    Staining