Sample amplification and labeling service

eagle-i ID

Resource Type

Properties

1. Fee for service

   Yes

2. Resource Description

   "Microarray DNA Variation Analysis: DNA Labeling Method - Affymetrix 250K - Affymetrix SNP 6.0 (Standard)"

3. Resource Description

   "Notes: - If the concentration of samples is unknown, please contact the Gene Microarray Shared Resource lab before submitting samples. - When submitting samples, RNA should be in an RNAse, DNAse-free 1.5ml microcentrifuge tube and in aqueous solution, preferably nuclease-free water. - When submitting samples with small RNA inputs (5-100ng total), please contact the Gene Microarray Shared Resource lab before submitting samples for any special considerations. - For any sample preparation and sample submission questions, contact the Gene Microarray Shared Resource lab.Reduced pricing is available for batch processing of 24 or more samples. - To submit polyA+ RNA for expression profiling, please contact the Gene Microarray Shared Resource lab for submission requirements."

4. Resource Description

   "RNA profiling: * 3'IVT Labeling method - Ambion MessageAMP Premier (Standard) - Affymetrix One Cycle cDNA synthesis - NuGEN Ovation Labeling * Whole Transcript Labeling for Gene and Exon arrays - Ambion Whole Transcript Sense target labeling (Standard) * miRNA labeling for Affymetrix miRNA array - Genisphere High Sensitivity FlashTag Biotin labeling"

5. Related Resource

   Affymetrix GeneChip System

6. Service Provided by

   Gene Profiling Shared Resource Core Laboratory

7. Related Protocol(s)

   Affymetrix microarray core labeling methods standard labeling assay

8. Related Technique

   Chemical labeling